Frozen radical prostatectomy samples (n = 5) were obtained from the University Tissue and Data Acquisition and Analysis Core after informed written consent and following approved Virginia Commonwealth University Institutional Review Board (IRB) protocols (HM13417_CR1). Each sample was reviewed and scored by a board certified pathologist with expertise in prostate cancer diagnosis. Frozen tissue slices (8 μm) from the biopsy cores were placed on uncharged glass slides, and stained with hematoxylin and eosin (H&E) using a standard protocol. Laser capture microdissection (LCM) was performed using the Arcturus Veritas™ laser capture microdissection system (Life Technologies). Each tissue type (benign, tumour) was separately captured onto CapSure® Macro LCM caps (Life Technologies). At least ten slides were captured for each patient included in the study. Total RNA was isolated from LCM caps using the ARCTURUS® PicoPure® RNA Isolation Kit (Life Technologies), following the manufacturers protocol. RNA quality and quantity were estimated using an Agilent RNA 6000 Pico chip with the Bioanalyzer 2100 (Agilent Technologies) using the manufacturer’s instructions.
Capsure macro lcm cap
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CapSure Macro LCM Caps are a specialized laboratory equipment designed for laser capture microdissection (LCM) applications. These caps are used to capture and isolate specific cells or tissue sections from a sample for further analysis.
Automatically generated - may contain errors
Lab products found in correlation
Picopure rna isolation kit, by Thermo Fisher Scientific (8 mentions)
Pen membrane glass slides, by Thermo Fisher Scientific (4 mentions)
Cresyl violet acetate, by Merck Group (3 mentions)
Arcturusxt laser capture microdissection system, by Thermo Fisher Scientific (3 mentions)
Rneasy micro kit, by Qiagen (3 mentions)
Superfrost plus glass slide, by Thermo Fisher Scientific (3 mentions)
Palm microdissection instrument, by Zeiss (2 mentions)
2100 bioanalyzer, by Agilent Technologies (2 mentions)
Mirneasy ffpe kit, by Qiagen (2 mentions)
Bioanalyzer rna pico chip, by Agilent Technologies (2 mentions)
Pixcell iie laser capture microdissection system, by Arcturus (2 mentions)
Cresyl violet, by Thermo Fisher Scientific (2 mentions)
Quantstudio real time pcr system, by Thermo Fisher Scientific (2 mentions)
High sensitivity rna screentape, by Agilent Technologies (2 mentions)
Cm 1950 microtome, by Thermo Fisher Scientific (2 mentions)
Arcturusxt, by Thermo Fisher Scientific (2 mentions)
Iscript reverse transcriptase kit, by Bio-Rad (2 mentions)
Ssoadvanced preamp supermix, by Bio-Rad (2 mentions)
Itaq universal sybr green supermix, by Bio-Rad (2 mentions)
Tapestation 4200, by Agilent Technologies (2 mentions)
39 protocols using capsure macro lcm cap
1
Prostate Cancer Tissue Profiling
2
Colorectal Adenocarcinoma Identification via LCM
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Eight μm tissue slides were generated and stained by LCM Staining Kit (Ambion) to identify colorectal adenocarcinoma foci versus normal glandular epithelium. LCM was performed using an Arcturus Veritas™ laser capture microdissection system (Life Technologies). Areas of interest were individually captured onto CapSure® Macro LCM caps (Life Technologies, Grand Island, NY). Total RNA was extracted from LCM captured material using Pure Link RNA Mini Kit (Ambion).
3
Laser Capture Microdissection of Hippocampal Neurons
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Laser capture microdissection (LCM) was performed using a PixCell IIe laser capture microscope with an infrared diode laser (Life Technologies, CA). Using serial sections immediately adjacent (before and after) to the miR-15b ISH/FJC labeled slides, we identified dying, Fluoro-Jade positive neurons and adjacent surviving, Fluoro-Jade negative neurons and laser captured them from the ipsilateral (directly under the injury site) rat hippocampus. Contamination from adjacent cells was minimized by using the smallest laser spot size (7.5 micron) and a power setting range of 75–100 mW with pulse duration of 0.45–0.85 ms, the last two settings adjusted as necessary for optimum capture. Surviving and dying neurons were separately captured on thermoplastic films of CapSure Macro LCM Caps (Life Technologies, CA). Caps were then secured on 0.5 mL tubes with 100 μL lysis solution from the RNAqueous-MicroRNA isolation kit (Ambion, Austin, TX) and vortexed for 15 sec, stored at −20°C, and vortexed 30 sec before the RNA isolation procedure.
4
Laser Capture Microdissection of Frozen Cerebellar Tissue
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Fresh frozen cerebella were sectioned at 10 µm onto Superfrost microscope slides (Fisher Scientific) and placed immediately on dry ice before storage at −80°C for no more than 5 days before microdissection. Sections were stained and dehydrated by passing through RNase-free coplin jars with solutions made in DEPC-treated distilled water, as follows: 30 s in 75% ethanol, 30 s in distilled water, 2 min in 1% toluidine blue in distilled water, 30 s in distilled water, 30 s in 75% ethanol, 30 s in 95% ethanol, 1 min in 100% ethanol (2 times), and 5 min in xylene. All staining solutions except xylene contained RNase inhibitor (Sigma R7397). Slides were immediately microdissected using the ArcturusXT laser capture microdissection system (Life Technologies) in infrared mode, according to the manufacturer’s instructions. Six to 10 sections from each lesion were captured onto CapSure macro LCM caps (Life Technologies), transferred immediately to RLT plus lysis buffer (Qiagen) with β-mercaptoethanol, briefly vortexed, and stored on dry ice until RNA extraction.
5
VTA Microdissection and Staining Protocol
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The VTA was collected bilaterally from three 14-μm coronal sections per animal on Superfrost plus glass slides. Brain sections were stained for 1 min with 0.1% cresyl violet acetate (Sigma-Aldrich, Brøndby, Denmark) dissolved in 70% EtOH. Sections were subsequently dehydrated briefly in 96% and 99.9% EtOH and finally dried at room temperature for at least 2 min. Using a PALM Microdissection instrument (Zeiss Microsystems, Jena, Germany), the VTA was identified and captured on Capsure Macro LCM Caps (Life Technologies, Applied Biosystems, Carlsbad, CA, USA).
6
Laser Capture Microdissection of Diabetic Foot Ulcer Epidermis
7
Laser-Captured Microanatomic Characterization of FMDV RNA
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Pharyngeal mucosal tissue samples selected on the basis of prescreening by qRT-PCR and VI were processed using laser capture microdissection (LCM) to determine the microanatomic distribution of FMDV RNA as previously described (30 (link)). In brief, four samples representing distinct microanatomic structures (nonlymphoid epithelium [Epith], lymphoid follicle-associated epithelium [FAE], mucosa-associated lymphoid follicles [LF], and submucosa [SM]) were dissected from 10-μm cryosections using an Arcturus XT LCM system. Dissected samples of 150,000 μm2 in surface area were captured onto individual CapSure Macro LCM caps (catalog number LCM0211; Life Technologies), which were immediately mounted onto microtubes containing 50 μl of RNA extraction buffer (PicoPure). RNA extraction was performed using a PicoPure RNA isolation kit (catalog number KIT0202; Life Technologies) with a final elution volume of 17 μl.
8
Laser-Capture Microdissection of Nasopharyngeal Tissue
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Nasopharyngeal tissue samples from either the dorsal nasopharynx or dorsal soft palate, were selected for laser-capture microdissection (LCM) based upon detection of FMDV by qRT-PCR and VI. The LCM procedure was performed as previously described11 (link),18 (link) with minor modifications. In brief, distinct samples consisting of follicle-associated epithelium (FAE) were dissected from 10 µm cryosections from each selected tissue sample using an Arcturus XT™ LCM system. Dissected samples with a combined surface area of approximately 400 000 µm2 were captured onto individual CapSure Macro LCM caps (LCM0211, Life technologies) which were immediately mounted onto micro-tubes containing 50 µl of RNA extraction buffer (PicoPure™). RNA extraction was performed using the PicoPure™ RNA isolation kit (KIT0202, Life Technologies) with a final elution volume of 12 µl. FMDV RNA content in micro-dissected tissue samples was determined by qRT-PCR as previously described11 (link). All micro-dissected FAE samples from transitional carriers and carriers were confirmed to contain FMDV RNA whereas samples from transitional terminators and non-carriers were FMDV-negative.
9
Epidermal miRs in Epithelial Neoplasms
10
Cresyl Violet Staining and Laser Capture of BL-d
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Brain sections from Ghr-R overexpressing mice subjected to restraint stress were stained for 30 seconds with 0.1% cresyl violet acetate (Sigma-Aldrich) solution dissolved in 70% EtOH. Sections were subsequently dehydrated briefly in 96% and 99.9% EtOH and finally dried at room temperature for at least 2 minutes. Using a Zeiss P.A.L.M. Microdissection instrument, the BL-d was identified and captured on CapSure Macro LCM Caps (Life Technologies, Applied Biosystems, Carlsbad, CA). BL-d was collected bilaterally from two 14-μm coronal sections per animal.
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