Sections of splenic tissue (3 μm) were prepared after overnight fixation in 10% formalin and embedding in paraffin. Sections were stained with H&E for morphologic evaluation. Primary antibodies, rabbit anti-mouse CD3 (clone: SP7; Thermo Fisher Scientific), rabbit anti-GFP (Molecular Probes, Invitrogen), or rabbit anti-mouse BCL6 (clone: N-3; Santa Cruz) or alkaline peroxidase (AP)-conjugated anti-mouse IgM and IgG1-antibodies (Southern Biotech) were applied to tissue sections and incubated overnight at 4°C. Secondary staining with anti-rabbit HRP-labeled polymer (Dako) was performed for BCL6, CD3 and eGFP and developed in aminoethylcarbazole (AEC; Sigma), while AP-conjugated antibodies were developed in nitro blue tetrazolium chloride-5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP; Roche). Sections stained for BCL6/IgG1 were counterstained with hematoxylin. Images were acquired via a Digital Sight camera mounted to a Nikon Eclipse E600 microscope (Nikon).
Rabbit anti mouse bcl6
Manufactured by Santa Cruz Biotechnology
Rabbit anti-mouse BCL6 is a primary antibody that recognizes the B-cell lymphoma 6 (BCL6) protein in mouse samples. BCL6 is a transcriptional repressor that plays a key role in the regulation of germinal center B-cell development and function.
Automatically generated - may contain errors
Lab products found in correlation
Eclipse e600 microscope, by Nikon (2 mentions)
Nbt bcip, by Roche (2 mentions)
Alkaline peroxidase, by Southern Biotech (2 mentions)
Labeled polymer hrp anti rabbit, by Agilent Technologies (2 mentions)
Rabbit anti gfp, by Thermo Fisher Scientific (2 mentions)
Rabbit anti mouse cd3, by Thermo Fisher Scientific (2 mentions)
Ap conjugated anti mouse igm and igg1 antibodies, by Southern Biotech (2 mentions)
2 protocols using rabbit anti mouse bcl6
1
Immunohistochemical Profiling of Splenic Tissues
2
Immunohistochemical Profiling of Splenic Tissues
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Sections of splenic tissue (3 μm) were prepared after overnight fixation in 10% formalin and embedding in paraffin. Sections were stained with H&E for morphologic evaluation. Primary antibodies, rabbit anti-mouse CD3 (clone: SP7; Thermo Fisher Scientific), rabbit anti-GFP (Molecular Probes, Invitrogen), or rabbit anti-mouse BCL6 (clone: N-3; Santa Cruz) or alkaline peroxidase (AP)-conjugated anti-mouse IgM and IgG1-antibodies (Southern Biotech) were applied to tissue sections and incubated overnight at 4°C. Secondary staining with anti-rabbit HRP-labeled polymer (Dako) was performed for BCL6, CD3 and eGFP and developed in aminoethylcarbazole (AEC; Sigma), while AP-conjugated antibodies were developed in nitro blue tetrazolium chloride-5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP; Roche). Sections stained for BCL6/IgG1 were counterstained with hematoxylin. Images were acquired via a Digital Sight camera mounted to a Nikon Eclipse E600 microscope (Nikon).
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