Anti ddx5

Manufactured by Fortis Life Sciences

Anti-DDX5 is a lab equipment product designed to detect and measure the presence of the DDX5 protein. DDX5 is an RNA helicase involved in various cellular processes. The core function of Anti-DDX5 is to facilitate the identification and quantification of DDX5 levels in biological samples. No further interpretation or extrapolation on the intended use of this product is provided.

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Lab products found in correlation

Anti rna pol 2, by Active Motif (2 mentions) Sybr green 1 master mix, by Roche (2 mentions) Anti h3k4me3, by Abcam (2 mentions) Anti flag tag, by Merck Group (2 mentions) Anti ha tag, by Cell Signaling Technology (2 mentions) Light cycler 48011, by Roche (1 mentions) Lightcycler 480 2, by Roche (1 mentions) Ab273434, by Abcam (1 mentions) Anti ha antibody 3f10, by Roche (1 mentions) Slowfade gold antifade reagent, by Thermo Fisher Scientific (1 mentions) Lab tek 2 well chamber slide, by Thermo Fisher Scientific (1 mentions) Anti ddx6 a300 460a, by Fortis Life Sciences (1 mentions) Anti ddx21 a300 627a, by Fortis Life Sciences (1 mentions) Anti g3bp1 a302 033a, by Fortis Life Sciences (1 mentions) Alexa fluor 594 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Gtx632269, by GeneTex (1 mentions) Fv1200, by Olympus (1 mentions)

3 protocols using anti ddx5

Chromatin Immunoprecipitation with qPCR Analysis

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Example 13

Conventional ChIP-qPCR was performed. Briefly, cells were crosslinked with 1% formaldehyde at room temperature for 10 minutes followed by glycine quenching, cell lysis, nuclei isolation and lysis, and sonication to obtain 150-200 bp DNA fragments. Complexes were immunoprecipitated overnight using 10 μg of anti-HA tag (Cell Signaling 3724), anti-Flag tag (Sigma F1804), anti-H3K4me3 (AbCam ab8580), anti-RNA Pol-II (Active Motif 61083), or anti-DDX5 (Bethyl A300-523A). Real-time qPCR of purified DNA was performed using SYBR Green I MasterMix (Roche 4707516001) on the Light Cycler 48011 (Roche). qPCR primers are provided in Table 9.

US11293018B2. Manipulation of nuclear architecture (2022-04-05). THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY [US], SYSTEM BIOSCIENCES, INC. [US]. Inventors: Kevin Chun-Kai Wang [US], Stefanie Morgan [US], Fangting Wu [US], Chiao-Chain Huang [US].

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Chromatin Immunoprecipitation qPCR Protocol

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Conventional ChIP-qPCR was performed. Briefly, cells were crosslinked with 1% formaldehyde at room temperature for 10 min followed by glycine quenching, cell lysis, nuclei isolation and lysis and sonication to obtain 150–200 bp DNA fragments. Complexes were immunoprecipitated overnight using 10 μg of anti-HA tag (Cell Signaling 3724), anti-Flag tag (Sigma F1804), anti-H3K4me3 (AbCam ab8580), anti-RNA Pol-II (Active Motif 61083) or anti-DDX5 (Bethyl A300-523A). Real-time qPCR of purified DNA was performed using SYBR Green I MasterMix (Roche 4707516001) on the Light Cycler 480II (Roche). qPCR primers are provided in Supplementary Table 1.

Morgan S.L., Mariano N.C., Bermudez A., Arruda N.L., Wu F., Luo Y., Shankar G., Jia L., Chen H., Hu J.F., Hoffman A.R., Huang C.C., Pitteri S.J, & Wang K.C. (2017). Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping. Nature Communications, 8, 15993.

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Immunofluorescence Staining of Cellular Markers

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Cells were grown on Lab-Tek 2-well chamber slides (Nunc, Thermo) at 2 × 10⁴ cells per well. The cells were fixed in 3.6% formaldehyde in phosphate-buffered saline (PBS), permeabilized in 0.1% NP-40 in PBS at room temperature, and incubated with anti-HA antibody (3F10; F. Hoffmann-La Roche AG, Basel, Switzerland) at a 1:300 dilution in PBS containing 3% bovine serum albumin (BSA) at 37°C for 30 min. I also used anti-DDX6 (A300-460A; Bethyl Lab), anti-XRN1 (A300-443A; Bethyl Lab), anti-G3BP1 (A302-033A; Bethyl), anti-DDX21 (A300-627A; Bethyl Lab), anti-MOV10 (A301-571A; Bethyl Lab), anti-DDX5 (A300-523A; Bethyl Lab), and anti-SARS-CoV-2 nucleocapsid (ab273434 [6H3]; Abcam or GTX632269 [6H3]; GeneTex) antibodies as primary antibodies. Cells were then stained with donkey anti-mouse IgG (H+L) Alexa Fluor 594-conjugated secondary antibody and/or donkey anti-rabbit or anti-rat IgG (H+L) Alexa Fluor 594-conjugated secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA), at a 1:300 dilution in PBS containing 3% BSA at 37°C for 30 min. Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole). Following washing 3 times in PBS, the cover slides were mounted on slides using SlowFade Gold antifade reagent (Life Technology). Samples were analyzed under a confocal laser-scanning microscope (FV1200; Olympus, Tokyo, Japan).

, & Ariumi Y. (2022). Host Cellular RNA Helicases Regulate SARS-CoV-2 Infection. Journal of Virology, 96(6), e00002-22.

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