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Clone bb7

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Clone BB7.2 is a monoclonal antibody that recognizes the human HLA-A2 antigen. It can be used for the identification and enumeration of HLA-A2-positive cells by flow cytometry.

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Lab products found in correlation

Lsrfortessa cell analyzer, by BD (2 mentions) Clone w6 32, by BioLegend (2 mentions) Clone ip26, by BioLegend (1 mentions) Quantibrite beads, by BD (1 mentions) Mc57g, by BioLegend (1 mentions)

4 protocols using clone bb7

1

Quantifying T Cell Surface Markers

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T cell receptor, CD8α, CD8β, and pMHC surface expression analyses were performed as previously described using PE-conjugated antibodies, and PE-labeled standardized beads (quantibrite beads, BD biosciences) (11 (link)). For TCR, CD8α, CD8 β, and pMHC the following PE-conjugated antibodies were used: clone IP26, Biolegend; clone SK1, Biolegend; clone 2ST8.5H7, Beckman Coulter; and clone BB7.2, Biolegend.
Williams C.M., Schonnesen A.A., Zhang S.Q., Ma K.Y., He C., Yamamoto T., Eckhardt S.G., Klebanoff C.A, & Jiang N. (2017). Normalized Synergy Predicts That CD8 Co-Receptor Contribution to T Cell Receptor (TCR) and pMHC Binding Decreases As TCR Affinity Increases in Human Viral-Specific T Cells. Frontiers in Immunology, 8, 894.
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2

Osteosarcoma Cell MHC Class I Expression

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Five osteosarcoma cell lines were stained with antibodies which is pan-reactive for MHC class I (clone W6/32, BioLegend Cat. 311405) or specific for HLA*A2 (clone BB7.2, BioLegend, Cat. 343308). Flow cytometry was performed using BD LSRFortessa cell analyzer (BD Biosciences).
Brohl A.S., Sindiri S., Wei J.S., Milewski D., Chou H.C., Song Y.K., Wen X., Kumar J., Reardon H.V., Mudunuri U.S., Collins J.R., Nagaraj S., Gangalapudi V., Tyagi M., Zhu Y.J., Masih K.E., Yohe M.E., Shern J.F., Qi Y., Guha U., Catchpoole D., Orentas R.J., Kuznetsov I.B., Llosa N.J., Ligon J.A., Turpin B.K., Leino D.G., Iwata S., Andrulis I.L., Wunder J.S., Toledo S.R., Meltzer P.S., Lau C., Teicher B.A., Magnan H., Ladanyi M, & Khan J. (2021). Immuno-transcriptomic profiling of extracranial pediatric solid malignancies. Cell reports, 37(8), 110047.
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3

Osteosarcoma Cell MHC Class I Expression

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Five osteosarcoma cell lines were stained with antibodies which is pan-reactive for MHC class I (clone W6/32, BioLegend Cat. 311405) or specific for HLA*A2 (clone BB7.2, BioLegend, Cat. 343308). Flow cytometry was performed using BD LSRFortessa cell analyzer (BD Biosciences).
Brohl A.S., Sindiri S., Wei J.S., Milewski D., Chou H.C., Song Y.K., Wen X., Kumar J., Reardon H.V., Mudunuri U.S., Collins J.R., Nagaraj S., Gangalapudi V., Tyagi M., Zhu Y.J., Masih K.E., Yohe M.E., Shern J.F., Qi Y., Guha U., Catchpoole D., Orentas R.J., Kuznetsov I.B., Llosa N.J., Ligon J.A., Turpin B.K., Leino D.G., Iwata S., Andrulis I.L., Wunder J.S., Toledo S.R., Meltzer P.S., Lau C., Teicher B.A., Magnan H., Ladanyi M, & Khan J. (2021). Immuno-transcriptomic profiling of extracranial pediatric solid malignancies. Cell reports, 37(8), 110047.
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4

AAV9-Mediated Cytokine Production and Expression

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The production of human cytokines, encoded by AAV9 vectors, was determined by infecting a murine macrophage cell line, MC57G (CRL2295TM - American Type Culture Collection, Manassas, VA), with the AAV9 vectors, followed by collecting the culture supernatant and performing ELISAs. Briefly, one day after plating MC57G cells in tissue culture plates, cells were infected with serially diluted AAV9 vectors encoding the respective human genes. Forty-eight hours after infection with AAV9 encoding each of the human cytokines, secretion of GM-CSF, IL-3, IL-4, IL-7, and IL-15 into the culture supernatant was determined using ELISAs [43] (link). Similarly, 48 hours after infecting MC57G cells with AAV9-A2, the level of A2 expression by the transduced MC57G cells was determined by FACS, using antibodies against HLA-A2 (clone BB7.2 - BioLegend, San Diego, CA) and hβ2m (Clone 2M2 - BioLegend)[35] (link), [44] (link).
Huang J., Li X., Coelho-dos-Reis J.G., Wilson J.M, & Tsuji M. (2014). An AAV Vector-Mediated Gene Delivery Approach Facilitates Reconstitution of Functional Human CD8+ T Cells in Mice. PLoS ONE, 9(2), e88205.
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