Trichrome

Manufactured by Polysciences

Sourced in United States

Trichrome is a versatile laboratory equipment used for the staining and visualization of various biological samples. It is designed to simultaneously stain multiple components within a sample, such as nuclei, cytoplasm, and connective tissues, with different colors. The Trichrome equipment enables clear differentiation and identification of these components, making it a valuable tool for researchers and clinicians in fields like histology, pathology, and microscopy.

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Lab products found in correlation

Neutral buffered formalin, by Avantor (1 mentions) Tissue plus o c t, by Thermo Fisher Scientific (1 mentions) Cryostar nx70, by Thermo Fisher Scientific (1 mentions) Mitosox, by Nikon (1 mentions) Eclipse ts2 fluorescent microscope, by Nikon (1 mentions) Vs120 virtual slide system, by Olympus (1 mentions) Periostin, by Abcam (1 mentions) Picrosirius red, by Polysciences (1 mentions) Ds ri2, by Nikon (1 mentions) Illustrator cs6, by Adobe (1 mentions) Eclipse ti2 inverted microscope, by Nikon (1 mentions)

3 protocols using trichrome

Histological Analysis of Harvested Organs

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Harvested organs were immediately placed in 10 mL of 10% (v/v) neutral buffered formalin (VWR, Radnor, PA, USA) and kept on ice until transferred to a 4 °C cold room, where organs were fixed overnight on a rocker. Organs were rinsed with 1× PBS, pH 7.4, for 10 min (3×) before embedding in paraffin or Tissue-Plus OCT (Thermo Fisher Scientific, Waltham, MA, USA). For histology, organs were cut in 6 µm serial sections using a CryoStar NX70 (Thermo Fisher Scientific, Waltham, MA, USA) and stained with MitoSox or H&E (Thermo Fisher Scientific, Waltham, MA, USA), or Trichrome (Polysciences, Warrington, PA, USA). Histological differences were quantified using ImageJ software. H&E and Trichrome images were collected by an Olympus VS120 virtual slide system (Olympus Life Sciences, Waltham, MA, USA). MitoSox images were collected with a Eclipse Ts2 fluorescent microscope (Nikon, Melville, NY, USA). Mean technical replicates (3–6 per parameter) were used to represent effects on a single heart. A minimum of three hearts were used for all quantified parameters. Protein quantification experiments used corresponding organs from seven different wildtype male, wildtype female, p66Shc^−/+ male, or p66She^−/+ female fish for a total of N = 7 per quantification.

Haslem L., Hays J.M., Zhang X.A, & Hays F.A. (2022). P66Shc (Shc1) Zebrafish Mutant Line as a Platform for Testing Decreased Reactive Oxygen Species in Pathology. Journal of Cardiovascular Development and Disease, 9(11), 385.

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Antibody-based Staining Techniques

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Reagents and chemicals were purchased of the highest grade available from Sigma-Aldrich (St.Louis, MO) unless otherwise noted. Primary and secondary antibodies were from various companies as indicated throughout. TriChrome and PAS staining kits were purchased from Polysciences (Warrington, PA).

Wicks S.E., Nguyen T.T., Breaux C., Kruger C, & Stadler K. (2015). Diet-induced obesity and kidney disease – in search of a susceptible mouse model. Biochimie, 124, 65-73.

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Histological and Immunostaining Analysis

Cited 1 time

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Tissues were stained with trichrome and picrosirius Red (Poly-sciences), for histologic examination. Tissues were evaluated using immunohistochemistry (IHC) and fluorescent microscopy as previously described [30 (link)]. Antibodies used for IHC and immunofluorescence included α-smooth muscle actin (SMA) and S100B (GeneTex), fibrinogen (Exalpha Biologicals), platelet CD41 (gift from Richard Aster; Versiti), periostin (Abcam), IBA-1 (Waco Chemicals USA), CD45 (R&D Systems), and Cre recombinase protein (Novus Biologicals). Appropriate fluorochrome conjugated anti-goat, anti-mouse, and anti-rabbit secondary antibodies were used for immunodetection (10 μg/mL) (Jackson ImmunoResearch Laboratories). The Easy Scan imaging system (Motic) scanned IHC slides. A Nikon Eclipse Ti2 inverted microscope with a DS-Ri2 high-speed color camera using a low-magnification 10×/0.45, 20×/0.75, 40×/0.95 or a high-magnification 100× oil/1.45 numerical aperture objective (Nikon Instruments) acquired immunofluorescent images. Images were analyzed with the Nikon NIS-Elements software platform and processed with Imaris multichannel microscopy software (Bitplane). Image formatting was performed in Adobe Photoshop CS-6 and Adobe Illustrator CS-6 (Adobe).

Maroney S.A., Siebert A.E., Martinez N.D., Rasmussen M., Peterson J.A., Weiler H., Lincoln J, & Mast A.E. (2022). Platelet tissue factor pathway inhibitor-α dampens cardiac thrombosis and associated fibrosis in mice. Journal of thrombosis and haemostasis : JTH, 21(3), 639-651.

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