The following antibodies were used at the indicated dilutions: c‐Myc (9E10, sc‐40, 1:1,000, Santa Cruz Biotechnology), Separase (A302‐215A, 1:2,000, Bethyl Laboratories), KIF4A (A301‐074A, 1:1,000, Bethyl Laboratories), Axin1 (#2087, 1:1,000, Cell Signaling Technology), B56α (610615, 1:3,000, BD Biosciences), BubR1 (A300‐995A,1:1,000, Bethyl Laboratories), PP2A catalytic subunit (05‐421, 1:2,000, Millipore), PP2A scaffold subunit (#2041, 1:1,000, Cell Signaling Technology) GFP (ab290, 1:4,000, Abcam), FoxO3 pS413 (#8174, 1:1,000, Cell Signaling Technology), FoxO3 rabbit polyclonal α‐pS253 (Raised against peptide CAPRRRAV(pS)MDNS; 1:500, Moravian Biotechnology), Anti‐RFP (1:1,000; MBL, FM005), Anti‐GFP (1:1,000; Roche, 11814460001), Rabbit anti‐ADAM17 (1:1,000, Abcam, 2051), Rabbit anti‐ADAM17 (1:1,000, Abcam, 39162), Rabbit anti‐EGFR (1:1,000, cell signaling, 2232), Rabbit anti‐pEGFR Y1068 (1:1,000, cell signaling, 2234), Mouse anti‐Transferrin receptor (1:1,000, Invitrogen, 136800), Mouse anti‐GAPDH (1:5,000, Sigma, G8795), rabbit Anti‐GFP (1:3,000, Takara Bio Clontech, 632592), Donkey anti‐rabbit‐HRP (1:2,000, GE Healthcare, NA934), Sheep anti‐mouse‐HRP (1:2,000, GE Healthcare, NXA931), and H3pS10 (06‐570, 1:1,000, Millipore).
Mouse anti transferrin receptor
Manufactured by Thermo Fisher Scientific
Sourced in United States
The mouse anti-transferrin receptor is a monoclonal antibody that binds to the transferrin receptor on the surface of cells. The transferrin receptor is responsible for the cellular uptake of iron-bound transferrin. This antibody can be used to detect and quantify the expression of the transferrin receptor in various cell types and biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti β actin, by Santa Cruz Biotechnology (3 mentions)
Mouse anti actin, by Merck Group (3 mentions)
Rabbit anti tace adam17, by Abcam (2 mentions)
Rabbit anti ha, by Santa Cruz Biotechnology (2 mentions)
Mouse anti ha, by Enzo Life Sciences (2 mentions)
Mouse anti gapdh, by Merck Group (2 mentions)
Mouse anti β actin, by Merck Group (2 mentions)
Rabbit anti erk1 2, by Cell Signaling Technology (2 mentions)
Rabbit anti egfr, by Merck Group (2 mentions)
Hrp coupled secondary antibody, by Jackson ImmunoResearch (2 mentions)
Image lab 5, by Bio-Rad (2 mentions)
Rabbit anti cav 1, by Abcam (2 mentions)
Criterion xt 4 12 bis tris gel, by Bio-Rad (2 mentions)
Rabbit anti connexin 43 antibody, by Thermo Fisher Scientific (2 mentions)
Mouse anti phospho caveolin1 py14, by BD (2 mentions)
Dc protein assay, by Bio-Rad (2 mentions)
Rabbit anti β catenin, by Abcam (2 mentions)
Ecl plus, by GE Healthcare (2 mentions)
Axin1, by Cell Signaling Technology (1 mentions)
Rabbit anti egfr, by Cell Signaling Technology (1 mentions)
19 protocols using mouse anti transferrin receptor
1
Antibody Validation for Western Blotting
2
Immunoblotting Antibodies and Detection
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The following antibodies were used: Goat anti-mouse amphiregulin (R&D Systems, catalogue number AF989; 1:1000), Mouse anti-nonphospho-Tyr1173-EGFR (Millipore, catalogue number 05-484; 1:1000), mouse anti-beta-actin (Santa Cruz, catalogue number sc-47778; 1:2000), rabbit anti-TACE/ADAM17 (Abcam, catalogue number ab39161; 1:2000), rabbit anti-HA (Santa-Cruz, catalogue number sc-805; 1:2000) and mouse anti-transferrin receptor (Invitrogen, catalogue number 13-6800; 1:1000). Corresponding species-specific HRP-coupled secondary antibodies were used from Santa Cruz and Cell Signaling.
3
Protein Expression Analysis in Tumor Samples
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Tumor samples were lysed in Laemmli buffer, proteins were separated by SDS-PAGE and immunoblotting was performed using the following antibodies: rabbit anti-collagen I (Millipore, Billerica, MA), mouse anti-transferrin receptor (Invitrogen), mouse anti-GAPDH (Millipore), rabbit anti-LTBP3 (Santa Cruz Biotechnology), rabbit anti-EGLN1 (Cell Signaling), the rabbit anti-actin antibody was generated in the laboratory. The rabbit anti-CYR61 antibody was a kind gift of Dr Lester Lau.
4
Subcellular Fractionation and Western Blot
5
Immunoblotting Protocol for Synaptic Proteins
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Immunoblotting was carried out as described previously (83 (link)). Primary antibodies used were rabbit anti-synaptotagmin I (Sigma-Aldrich, St. Louis, Missouri; cat.no. S 2177; 1:5000), rabbit anti-synaptophysin (Epitomics, Burlingame, California; cat.no. 1485–1; 1:2000), mouse anti-transferrin receptor (Invitrogen, Carlsbad, California; cat.no. 13–6800; 1:2000) and mouse anti-β-actin (Chemicon, Temecula, California; cat.no. MAB1501R; 1:40 000). Secondary antibodies were goat anti-mouse-HRP (Dako, Glostrup, Denmark; 1:20 000) and goat anti-rabbit-HRP (Bio-Rad, Hercules, California; 1:20 000). Quantitative analysis was done by using the rectangle tool of the Image Lab analysis software (version 5.2.1, Bio-Rad, Hercules, California).
6
Lipid and Antibody Reagent Protocol
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N-hexanoyl-d- erythro-sphingosine (d- cer-C6) and N-hexanoyl-l- erythro-sphingosine (l- cer-C6) obtained from Matreya were dissolved in pure ethanol (Merck) as stock solution. Cycloheximide was purchased from A.G. Scientific, rapamycin was obtained from EMD Millipore, and both were dissolved in DMSO as stock solutions. Sheep anti–human TGN46 was obtained from AbD Serotec. Goat anti-GRASP65 (C-20) was obtained from Santa Cruz Biotechnology, Inc. Mouse anti–β-actin (clone AC-15) was obtained from Sigma-Aldrich. Rabbit polyclonal antibody against GFP was purchased from Abcam. Mouse anti-p230, mouse anti-Sec31a, and mouse anti-EEA1 were obtained from BD. Mouse anti-M6PR (mannose-6 phosphate receptor) was obtained from Thermo Fisher Scientific, mouse anti–transferrin receptor was purchased from Invitrogen, and mouse anti-LAMP1 was obtained from Stressgen. Alexa Fluor–labeled secondary antibodies were obtained from Invitrogen, and HRP-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. Protein A–gold was obtained from the Department of Cell Biology at Utrecht University (Utrecht, Netherlands).
7
Antibody Validation for Western Blot and IF
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The following antibodies were used for western blotting (WB) and immunofluorescence (IF): mouse anti-beta-actin (Santa Cruz, catalog number sc-47778; WB 1:2000), mouse anti-HA (ENZO, catalog number ENZ-ABS120-0200; WB 1:1000), mouse anti-transferrin receptor (Invitrogen, catalog number 13–6800; WB 1:1000), rabbit anti-Stim1 (Cell Signaling Technology, catalog number 5668S (D88E10); WB 1:2000), rabbit anti-Orai1 (Sigma Aldrich, catalog number O8264; WB 1:2500), goat anti-myc tag (Abcam, catalog number ab9132; IF 1:2000), rabbit anti-RHBDL2 (Proteintech, catalog number 12467-1-AP; WB 1:250 – only detected RHBDL2 in HaCaT lysates), rabbit anti-V5 tag (Cell Signaling Technology, catalog number 13202S; WB and IF 1:2000). Corresponding species-specific HRP or fluorescently coupled secondary antibodies were used from Santa Cruz and Cell Signaling (WB) or Invitrogen (IF).
8
Immunodetection of membrane proteins
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The following antibodies and reagents were used: mouse anti-Na,K-ATPase β-subunit (clone M17-P5-F11) (Thermo Scientific, Rockford, IL, USA), rabbit anti-GFP (Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-Na,K-ATPase α-subunit (Merck Millipore, Darmstadt, Germany), rabbit anti-β-actin (Sigma Aldrich, St. Louis, MO, USA), mouse anti-transferrin receptor (Invitrogen, Rockford, IL, USA), rabbit anti-PDI (Cell Signaling, Danvers, MA, USA), rabbit anti-GM130 (Cell Signaling, Danvers, MA, USA), rabbit anti-calnexin (Abcam, Cambridge, UK), rabbit anti-BiP (Cell Signaling, Danvers, MA, USA), HRP-conjugated anti-mouse and anti-rabbit IgG (Cell Signaling, Danvers, MA, USA), and Alexa Fluor 488 and 594 conjugated anti-rabbit, anti-mouse (Thermo Scientific, Eugene, OR, USA). α-ketoglutaric acid was obtained from Sigma Aldrich, St. Louis, MO, USA.
9
Plasma Membrane Protein Isolation and Fractionation
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MinuteTM Plasma Membrane Protein Isolation and Cell Fractionation Kit (Invent, Plymouth, USA) were adopted to separate the total and plasma membrane KCNB1 proteins. We collected the total protein fractions before cellular component separation and isolated plasma membrane proteins according to the manufacturer's protocol. The proteins of CHO-K1 co-expressing variants and wild-type were extracted using ice-cold RIPA buffer with a ratio of 100:1 protease inhibitor cocktails (Beyotime, Shanghai, China). The total amount of plasmid DNA here maintained 4 ug.
20 ug of samples of proteins were separated by 10% sodium dodecyl sulfate– polyacrylamide (SDS-PAGE), transferred to PVDF membranes, blocked in protein free rapid blocking buffer (Epizyme, Shanghai, China), and incubated with mouse anti-Kv2.1 (1:1000; Abcam, ab192761), mouse anti-transferrin receptor (1:500; Invitrogen, #13-6800), rabbit anti-GAPDH antibody (1:2000; Servicebio, GB11002) overnight at 4 °C, then incubated with peroxidase-conjugated goat anti-rabbit IgG (1:10,000 Jackson ImmunoResearch) and goat anti-mouse IgG (1:10,000, Jackson ImmunoResearch) (1h at room temperature). Protein quantitation was completed using ImageJ software (NIH, Bethesda, USA).
20 ug of samples of proteins were separated by 10% sodium dodecyl sulfate– polyacrylamide (SDS-PAGE), transferred to PVDF membranes, blocked in protein free rapid blocking buffer (Epizyme, Shanghai, China), and incubated with mouse anti-Kv2.1 (1:1000; Abcam, ab192761), mouse anti-transferrin receptor (1:500; Invitrogen, #13-6800), rabbit anti-GAPDH antibody (1:2000; Servicebio, GB11002) overnight at 4 °C, then incubated with peroxidase-conjugated goat anti-rabbit IgG (1:10,000 Jackson ImmunoResearch) and goat anti-mouse IgG (1:10,000, Jackson ImmunoResearch) (1h at room temperature). Protein quantitation was completed using ImageJ software (NIH, Bethesda, USA).
10
Antibody Panel for Protein Analysis
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The following antibodies were used: mouse anti-beta-actin (Santa Cruz, catalogue number sc-47778; WB 1:2000), rabbit anti-TACE/ADAM17 (Abcam, catalogue number ab39161; WB 1:2000), rabbit anti-HA (Santa-Cruz, catalogue number sc-805; IF 1:1000), mouse anti-HA (ENZO, catalogue number ENZ-ABS120-0200; PM IP 1:1000), mouse anti-transferrin receptor (Invitrogen, catalogue number 13–6800; 1:1000), rabbit anti-phosphoserine (Invitrogen, catalogue number 618100; WB 1:1000), rat HA-HRP, clone 3F10 (Roche, catalogue number 12013819001; WB 1:2000), mouse anti-p97 (Pierce/Thermo, catalogue number MA1-21412; WB 1:1000), rabbit anti-pan14-3-3 (Cell Signalling, catalogue number 8312; WB 1:1000), mouse anti-GM130 (BD Transduction labs, catalogue number 610823; IF 1:1000), rabbit anti-pan-cadherin (Cell Signalling, catalogue number 4068S; WB 1:1000), rabbit anti-ADAM10 (Abcam, catalogue number ab1997; WB 1:1000), rabbit anti-ERK1/2 (Cell Signalling, catalogue number 9102, WB 1:1000), rabbit anti-pERK1/2 (Cell Signalling, catalogue number 4377, WB 1:1000). Corresponding species-specific HRP or fluorescently coupled secondary antibodies were used from Santa Cruz and Cell Signaling (WB) or Invitrogen (IF).
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