Ms 222 3 amino benzoic acid ethyl ester

Manufactured by Merck Group

Sourced in Germany

MS-222; 3-amino benzoic acid ethyl ester is a general anesthetic and analgesic compound commonly used in research applications. Its core function is to induce anesthesia and immobilization in animals, particularly in aquatic species such as fish, amphibians, and crustaceans. The product is available in various formulations and concentrations to meet the diverse needs of researchers and laboratory settings.

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Lab products found in correlation

Mz125, by Leica (1 mentions) Dfc295 digital camera, by Leica (1 mentions) Tricaine methanesulfonate, by Merck Group (1 mentions)

Spelling variants of this product

MS-222 (1416 citations) Benzoic acid (240 citations) Amino acids (83 citations)

3 protocols using ms 222 3 amino benzoic acid ethyl ester

Smurf Assay for Zebrafish Larvae

Cited 1 time

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Smurf assays were conducted according to Dambroise et al. [47 (link)] by placing zebrafish larvae in 50 mL Falcon tubes containing 2.5% blue #1 (erioglaucine disodium salt, Sigma) for 30 min at room temperature. They were then rinsed under aquarium water until no more blue coloration could be found in the eluate, anesthetized in 0.016% tricaine (MS-222; 3-amino benzoic acid ethyl ester, Sigma) and imaged on a bright-field stereomicroscope (Leica MZ125) equipped with a Leica DFC295 digital camera. After imaging, DNA was extracted for genotyping analyses.

Raby L., Völkel P., Hasanpour S., Cicero J., Toillon R.A., Adriaenssens E., Van Seuningen I., Le Bourhis X, & Angrand P.O. (2021). Loss of Polycomb Repressive Complex 2 Function Alters Digestive Organ Homeostasis and Neuronal Differentiation in Zebrafish. Cells, 10(11), 3142.

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Fish Euthanasia Protocol

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This study was carried out in strict accordance with the Protection of Animals Act Germany (status 07/07/2014). The protection was approved by the regional council Freiburg (reference number 35–9185.64/1.1). All fish were killed with an overdose of MS 222 (3-Aminobenzoic acid ethyl ester, concentration of 1 g/L water, Sigma-Aldrich, Steinheim, Germany).

Yohannes E., Grimm C., Rothhaupt K.O, & Behrmann-Godel J. (2017). The Effect of Parasite Infection on Stable Isotope Turnover Rates of δ15N, δ13C and δ34S in Multiple Tissues of Eurasian Perch Perca fluviatilis. PLoS ONE, 12(1), e0169058.

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Euthanasia of African Clawed Toads

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Nine adult African Clawed Toads, X. laevis (Daudin, 1802) (seven females: body weights: 18.8-203.0 g, snout-vent lengths: 60-115 mm; two males: body weights: 21.0-48.6 g, snout-vent lengths: 55-80 mm) were studied. All animals were killed by immersion in an aqueous solution (0.5%) of tricaine methanesulfonate (MS 222: 3-aminobenzoic acid ethyl ester; Sigma Chemicals).

Lametschwandtner A, & Minnich B. (2023). Ocular microvasculature in adult Xenopus laevis: Scanning electron microscopy of vascular casts. Journal of morphology, 284(3).

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