Gel filtration standards 151 1901

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Gel filtration standards (151-1901) are a set of protein molecular weight markers used to calibrate and standardize gel filtration chromatography columns. These standards provide a range of known molecular weights to help determine the molecular weight of unknown protein samples during gel filtration analysis.

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Lab products found in correlation

Anti b raf sc 5284, by Santa Cruz Biotechnology (1 mentions) Tgf β1, by R&D Systems (1 mentions) Amersham pharmacia biotech akta fplc system, by GE Healthcare (1 mentions)

2 protocols using gel filtration standards 151 1901

Dynein Intermediate Chain Antibody Detection

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Anti-dynein intermediate chain (DIC) (mAb 1685) was from Chemicon (Billerica, MA). Anti-phospho-p44/42MAPK (phospho Erk1/2; 4370S), anti-p44/42 MAPK (Erk1/2; 4780), anti-phospho-MEK1/2 (9121S), anti-phospho-JNK (9251S) were from Cell Signaling Technology (Beverly, MA). The mouse anti-Smad2 (610843) and the mouse p150^Glued Ab (610474) were from BD Biosciences Transduction Laboratories (San Jose, CA). Anti-B-Raf (sc-5284) was from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-pan-Ras (MAB5195) was from Millipore (Bedford, MA), whereas anti-RRas (C-8) (SC-166221) was from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit monoclonal DYNLL1 Ab (ab51603) was from Abeam (Cambridge, MA). TGFβ1 was purchased from R & D Systems (Minneapolis, MN). Gel filtration standards (151-1901) were purchased from Bio-Rad (Hercules, CA). Our km23-1 antibodies (Abs) (27-43 for Western blotting; 1-15 for immunofluorescence) were produced as previously described (Jin et al., 2007 (link)).

Raza A., Pandey M.S., Jin Q, & Mulder K.M. (2019). km23-1/DYNLRB1 regulation of MEK/ERK signaling and R-Ras in invasive human colorectal cancer cells. Cell biology international, 44(1), 155-165.

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Laccase Native Status Determination

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The native status was determined using gel filtration chromatography (GFC). The semi-purified protein was loaded onto a Sephacryl 200 (16/60) gel filtration column previously pre-equilibrated with K₂HPO₄ (50 mM, pH 7.35). The protein was eluted using the same equilibrium buffer at a flow rate of 0.3 mL/min, and the absorbance signal was monitored at 280 nm. Afterward, laccase activity was measured in each of the fractions using a standard reaction mixture. Gel filtration standards #151-1901 (Bio–Rad Laboratories, Hercules, CA, USA) were loaded on a Sephacryl 200 (16/60) gel filtration column using the same conditions for the laccase protein. The column was coupled to the Amersham Pharmacia Biotech AKTA FPLC system (GE Healthcare, Chicago, IL, USA) in both cases.

González-González P., Gómez-Manzo S., Tomasini A., Martínez y Pérez J.L., García Nieto E., Anaya-Hernández A., Ortiz Ortiz E., Castillo Rodríguez R.A., Marcial-Quino J, & Montiel-González A.M. (2023). Laccase Production from Agrocybe pediades: Purification and Functional Characterization of a Consistent Laccase Isoenzyme in Liquid Culture. Microorganisms, 11(3), 568.

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