Good s buffers

Nicotine amide adenine dinucleotide (NAD) disodium salt, 3-phosphoglycerate [D-(–)-3-phosphoglyceric acid disodium salt], l-homoserine, d-homoserine, l-homocysteine, 2-oxoglutarate (α-ketoglutaric acid sodium salt), oxaloacetate, cis-aconitate, molecular weight standards (Gel Filtration Markers Kit for Protein Molecular Weights) for size exclusion chromatography analyses, and Murashige–Skoog (MS) vitamin solution were purchased from Sigma–Aldrich Co., Ltd. (St. Louis, MO, USA). The other amino acids, the salt mixture of MS medium, Good’s buffers, NADH (β-diphosphopyridine nucleotide disodium salt, reduced form), fumarate, malate (l-malate), citrate (citric acid monohydrate), and isocitrate [trisodium ( ± )-isocitrate n-hydrate] were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).

Nicotine amide adenine dinucleotide (NAD) disodium salt, D-(−)-3-phosphoglyceric acid (3-PGA) disodium salt, and molecular weight marker for size exclusion chromatography analysis were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, United States). Molecular weight markers for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis were purchased from BioDynamics Laboratory Inc. (Tokyo, Japan). The salt mixture of Gamborg’s B5 medium and Good’s buffers were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).