Ifnar1ko

WT C57BL/6 and BALB/c mice were purchased from Charles River Laboratories. Rag1^KO, Ifng^KO, Ifnar1^KO, Klrk1^KO, Sting^KO, Batf3^KO, Ticam1^KO, Myd88^KO, Ccr5^KO, Cxcr3^KO, CD11c-DTR, and ROSA^DTR C57BL/6 mice were purchased from the Jackson Laboratory. Single-KO mice were bred to generate double-KO mice, including Ticam1^KO Myd88^KO, Ccr5^KO Cxcr3^KO, and Ifnar1^KO Cxcr3^KO. Ncr1^iCre mice (a gift from Eric Vivier, Aix Marseille University Hospital, Marseille, France) were bred with ROSA^DTR mice to generate Ncr1^iCre ROSA^DTR mice. MMTV-PyMt^tg mice (a gift from David DeNardo, Washington University, St. Louis, Missouri, USA) were maintained on BALB/c background. All mice were housed in pathogen-free facilities in accordance with the guidelines instituted by the animal study committees of Massachusetts General Hospital.

All procedures were performed in compliance with the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals and according to the University of Miami Institutional Animal Care and Use Committee (IACUC) approved protocols. Tnfr1, Ifnar1, and Gsdmd knockout animals (Tnfr1KO, Ifnar1KO, and GsdmdKO, respectively) and C57BL/6 J mice as the wild type controls were obtained from the Jackson Laboratory (Bar Harbor, Maine, United States; stock numbers 003242, 028288, 032663, 000664). Mice were housed under standard conditions of temperature and humidity, with a 12-h light to dark cycle and free access to food and water. All animals have the C57BL/6J genetic background. All methods were completed and reported in accordance with ARRIVE guidelines.