Log In Sign up
The largest database of trusted experimental protocols

Proteome profiler human phospho kinase arrays ary003b

Manufactured by R&D Systems
Visit Supplier

The Proteome Profiler Human Phospho-Kinase Arrays ARY003B is a membrane-based antibody array designed to detect the phosphorylation levels of 43 different kinase phosphorylation sites and 2 related total proteins. It provides a semi-quantitative measurement of the phosphorylation state of key cellular proteins involved in signal transduction pathways.

Automatically generated - may contain errors

Lab products found in correlation

100 mm plates, by BD (1 mentions) 150 mm plates, by Corning (1 mentions)

Close spelling variants of this product

Proteome Profiler Human Phospho-Kinase Array (ARY003B) Human Phospho-Kinase Array (Proteome Profiler Array, Proteome Profiler Human Phospho-Kinase Array Proteome Profiler™ Phospho-Kinase Array Proteome Profiler Human Phospho-Array Human phospho-kinase profiler array Proteome Profiler Human Array Human Phospho-Kinase Array Proteome Profiler Array Proteome Profiler

2 protocols using proteome profiler human phospho kinase arrays ary003b

1

Phospho-Kinase Array Analysis of VCaP Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
VCaP or VCaP/GanR were plated at 5×106 in 100 mm plates (BD Falcon) in media containing 10% FBS. The following day medium was replaced with DMEM containing 10% FBS and 500 nmol/L ganitumab or control IgG1 antibody and incubated for six hours prior to harvest. Proteome Profiler Human Phospho-Kinase Arrays ARY003B were obtained from R&D systems and performed following the manufacturer's protocol. Densitometry was performed using Adobe Photoshop CS3.
Fahrenholtz C.D., Greene A.M., Beltran P.J, & Burnstein K.L. (2014). A novel calcium-dependent mechanism of acquired resistance to IGF-1 receptor inhibition in prostate cancer cells. Oncotarget, 5(19), 9007-9021.
+ Open protocol
+ Expand
2

Phospho-Kinase Array Analysis of 22Rv1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
22Rv1 cells were plated at 3 × 106 in 150-mm plates (Corning) in medium containing 10% FBS. After 24-hour serum starvation, cells were pretreated with 1 μM relcovaptan or vehicle control, followed by treatment with 100 nM AVP or vehicle control. Samples of 200 μg of protein from each treatment group were subjected to Proteome Profiler Human Phospho-Kinase Arrays ARY003B (R&D Systems) following the manufacturer’s protocol. Densitometry was performed using Adobe Photoshop Elements 14.
Zhao N., Peacock S.O., Lo C.H., Heidman L.M., Rice M.A., Fahrenholtz C.D., Greene A.M., Magani F., Copello V.A., Martinez M.J., Zhang Y., Daaka Y., Lynch C.C, & Burnstein K.L. (2019). Arginine vasopressin receptor 1a is a therapeutic target for castration-resistant prostate cancer. Science translational medicine, 11(498), eaaw4636.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!