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Md lmd laser microdissecting microscope

Manufactured by Leica
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The MD LMD laser microdissecting microscope is a specialized laboratory equipment used for precise isolation of cells or tissue samples from a larger sample. It utilizes a laser beam to selectively cut and extract the target area of interest.

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Lab products found in correlation

Cells to ct kit, by Thermo Fisher Scientific (2 mentions) Polyethylene terephthalate slides, by Leica (2 mentions) Rneasy micro kit, by Qiagen (1 mentions) Bioanalyzer 2100 system, by Agilent Technologies (1 mentions)

Close spelling variants of this product

LMD microscope (2 citations)

3 protocols using md lmd laser microdissecting microscope

1

Hypoxic Tumor Tissue Analysis

Cited 1 time
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In vivo tumor samples from a previous study46 (link) injected with Hypoxyprobe (Hypoxyprobe Inc., Burlington, MA) were used for the present study. Frozen sections (10 μm) were affixed onto polyethylene terephthalate slides (Leica), fixed in cold acetone (10 minutes), washed in PBS 3 times, stained for Hypoxyprobe using FITC-conjugated antibody for 1 hour on top of ice, washed, and air-dried; dissection immediately followed. Microdissection was performed using an MD LMD laser microdissecting microscope (Leica). RNA was isolated from some samples using the Cells to Ct Kit (Applied Biosystems), according to manufacturer instructions. Real-time PCR was carried out to quantify gene expression.
Rupaimoole R., Wu S.Y., Pradeep S., Ivan C., Pecot C.V., Gharpure K.M., Nagaraja A.S., Armaiz-Pena G.N., McGuire M., Zand B., Dalton H.J., Filant J., Miller J.B., Lu C., Sadaoui N.C., Mangala L.S., Taylor M., van den Beucken T., Koch E., Rodriguez-Aguayo C., Huang L., Bar-Eli M., Wouters B.G., Radovich M., Ivan M., Calin G.A., Zhang W., Lopez-Berestein G, & Sood A.K. (2014). Hypoxia Mediated Downregulation of miRNA Biogenesis Promotes Tumor Progression. Nature communications, 5, 5202.
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2

Hypoxic Tumor Tissue Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
In vivo tumor samples from a previous study46 (link) injected with Hypoxyprobe (Hypoxyprobe Inc., Burlington, MA) were used for the present study. Frozen sections (10 μm) were affixed onto polyethylene terephthalate slides (Leica), fixed in cold acetone (10 minutes), washed in PBS 3 times, stained for Hypoxyprobe using FITC-conjugated antibody for 1 hour on top of ice, washed, and air-dried; dissection immediately followed. Microdissection was performed using an MD LMD laser microdissecting microscope (Leica). RNA was isolated from some samples using the Cells to Ct Kit (Applied Biosystems), according to manufacturer instructions. Real-time PCR was carried out to quantify gene expression.
Rupaimoole R., Wu S.Y., Pradeep S., Ivan C., Pecot C.V., Gharpure K.M., Nagaraja A.S., Armaiz-Pena G.N., McGuire M., Zand B., Dalton H.J., Filant J., Miller J.B., Lu C., Sadaoui N.C., Mangala L.S., Taylor M., van den Beucken T., Koch E., Rodriguez-Aguayo C., Huang L., Bar-Eli M., Wouters B.G., Radovich M., Ivan M., Calin G.A., Zhang W., Lopez-Berestein G, & Sood A.K. (2014). Hypoxia Mediated Downregulation of miRNA Biogenesis Promotes Tumor Progression. Nature communications, 5, 5202.
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3

Laser Microdissection of Ovarian Samples

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Microdissection and RNA isolation were performed as previously described [44 (link)]. Briefly, fibroblasts were identified by a pathologist and microdissected from 7 μm frozen sections of ovarian tumors or normal ovary using a MD LMD laser microdissecting microscope (Leica, Wetzlar, Germany). RNA was isolated immediately in RLT lysis buffer and was extracted and purified using the RNeasy Micro kit (Qiagen, Valencia, CA). All purified total RNA specimens were quantified and checked for quality with a Bioanalyzer 2100 system (Agilent, Palo Alto, CA). Total RNA amplification and hybridization were performed as previously described [44 (link)]. (GEO accession number GSE40595).
Moran-Jones K., Gloss B.S., Murali R., Chang D.K., Colvin E.K., Jones M.D., Yuen S., Howell V.M., Brown L.M., Wong C.W., Spong S.M., Scarlett C.J., Hacker N.F., Ghosh S., Mok S.C., Birrer M.J, & Samimi G. (2015). Connective tissue growth factor as a novel therapeutic target in high grade serous ovarian cancer. Oncotarget, 6(42), 44551-44562.
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