Standard 3 trimethylsilyl propionic 2 2 3 3 d4 acid sodium salt tmsp

Mini-PROTEAN^® TGX™ precast gels 4–20%, Precision Plus Protein™ Unstained Standards, Clarity™ Western ECL Substrate, and DC™ protein assay were bought from Bio-Rad Laboratories (Segrate, Milano, Italy). Ultra-low Endotoxin FBS was purchased from Euroclone (Pero, Milano, Italy). Rabbit anti-TLR4 polyclonal FITC-conjugated antibody was purchased from StressMarq. Rabbit monoclonal anti-NF-kB (#8242), rabbit monoclonal anti-p-NF-kB (#3031), rabbit monoclonal anti-NLRP3 (#15101), rabbit monoclonal anti-iNOS (#13120) or anti-rabbit IgG, HRP-linked (#7074) antibodies were purchased from Cell Signalling Technology (Danvers, MA, USA). ELISA kit for TNF-α quantification and Griess (G4410) reagent for nitrite quantification was from Sigma-Aldrich (Saint Louis, MO, USA).
Deuterium oxide (D₂O, 99.90% D) and CD₃OD (99.80% D) were purchased from Eurisotop (Cambridge Isotope Laboratories, Inc., Saint-Aubin, France). Lipopolysaccharide (LPS) from E. coli O127:B8, mouse anti-tubulin antibody, FITC-conjugated LPS from E. coli O111:B4, standard 3-(trimethylsilyl)-propionic-2,2,3,3-d4 acid sodium salt (TMSP), sodium phosphate dibasic anhydrous, sodium phosphate monobasic anhydrous, phosphate-buffered saline (PBS), Dulbecco’s phosphate-buffered saline (DPBS), Accutase and all other solvents and chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).

Deuterium oxide (D₂O, 99.90% D),
CD₃OD (99.80% D), and CDCl₃ with 0.03% TMS (99.80%
D) were purchased from Eurisotop (Cambridge
Isotope Laboratories, Inc., France). Standard 3-(trimethylsilyl)-propionic-2,2,3,3-d₄ acid sodium salt (TMSP), sodium phosphate
dibasic anhydrous, sodium phosphate monobasic anhydrous, and all the
other solvents and chemicals were purchased from Sigma-Aldrich Co.
(St. Louis, MO, USA).