All animal procedures were approved by the Institutional Animal Care and Use Committees of The Scripps Research Institute (TSRI). All animals were housed in the same vivarium conditions at a constant temperature of 20oC, with a 12 hour light/dark cycle.
Constitutive, RBC-specific, and macrophage specific GOF Piezo1 mice were generated by breeding mice with conditional GOF Piezo1 allele with Cmv-cre (The Jackson Laboratory stock# 006054), EpoR-cre (a gift from Dr. Klingmuller group at Max-Planck-Institute für Immunbiologie, Freiburg, Germany), and LysM-cre (The Jackson Laboratory stock# 004781). All GOF Piezo1 mice were generated at Taconic, Hudson, NY, and maintained on C57BL/6 background (Ma et al., 2018 ). Heterozygous GOF mice were used for the experiments, unless mentioned otherwise. Macrophage-specific LOF Piezo1 mice were generated by breeding mice with conditional LOF Piezo1 allele (The Jackson Laboratory stock# 029213) with the same LysM-cre. All cre driver mice were on C57BL/6 background or were backcrossed at least 10 generations to C57BL/6. Littermates with a mix of equal number of male and female mice were used for experiments. Aging mice (12months to 18months old) born from same breeders were used in the experiments.
Constitutive, RBC-specific, and macrophage specific GOF Piezo1 mice were generated by breeding mice with conditional GOF Piezo1 allele with Cmv-cre (The Jackson Laboratory stock# 006054), EpoR-cre (a gift from Dr. Klingmuller group at Max-Planck-Institute für Immunbiologie, Freiburg, Germany), and LysM-cre (The Jackson Laboratory stock# 004781). All GOF Piezo1 mice were generated at Taconic, Hudson, NY, and maintained on C57BL/6 background (Ma et al., 2018 ). Heterozygous GOF mice were used for the experiments, unless mentioned otherwise. Macrophage-specific LOF Piezo1 mice were generated by breeding mice with conditional LOF Piezo1 allele (The Jackson Laboratory stock# 029213) with the same LysM-cre. All cre driver mice were on C57BL/6 background or were backcrossed at least 10 generations to C57BL/6. Littermates with a mix of equal number of male and female mice were used for experiments. Aging mice (12months to 18months old) born from same breeders were used in the experiments.