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Rediject d luciferin ultra

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RediJect D-Luciferin Ultra is a bioluminescent substrate used for in vivo imaging applications. It is formulated to provide a stable, high-intensity signal for monitoring biological processes in living systems.

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Lab products found in correlation

Living image software, by PerkinElmer (2 mentions) Ivis spectrum in vivo imaging system, by PerkinElmer (2 mentions) Living image 4, by PerkinElmer (1 mentions) Ivis lumina 2 in vivo imaging system, by PerkinElmer (1 mentions)

Close spelling variants of this product

RediJect D-Luciferin Ultra Bioluminescent Substrate XenoLight RediJect D-Luciferin Ultra Rediject D-Luciferin Ultra Bioluminescence Substrate XenoLight RediJect D-Luciferin Ultra Bioluminescent Substrate RediJect D-Luciferin D-luciferin Luciferin

10 protocols using rediject d luciferin ultra

1

In Vivo Bioluminescence Imaging Protocol

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Example 15

In Vivo Bioluminescence Imaging.

In vivo Bioluminescence Imaging was conducted as previously described10. Specifically, 5×1010 and 2×1011 T4 or T4-AAV particles were intramuscularly injected into BALB/c mice. At 0.25 day (d), 0.5 d, 1 d, 2 d, 5 d, 10 d, 15 d, 30 d, and 60 d post-administration, 30 μg of RediJect D-Luciferin Ultra (Perkin-Elmer™, MA), a luciferase substrate dissolved in 0.9% saline, was injected intraperitoneally. After 5 min, the mice were lightly anesthetized with 2% isoflurane and placed on an IVIS 200 bioluminescence whole-body imaging workstation (Caliper™). The bioluminescence emission signal was quantified using the camera control program, Living Image software, and displayed in physical units of surface radiance, photons per second per centimeter squared per steradian (photons/second/cm2/sr).

US11155835B2. Prokaryotic-eukaryotic hybrid viral vector for delivery of large cargos of genes and proteins into human cells (2021-10-26). The Catholic University of America [US]. Inventors: Venigalla B. Rao [US], Jingen Zhu [US].
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2

Luciferase Assay for Wnt Signaling Analysis

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Stable cell lines expressing the TOP-FLASH reporter were generated by transducing
cells with 7TFP recombinant lentiviruses (Fuerer
and Nusse, 2010), and luciferase assay was performed as previously
described (Singh et al., 2012 (link)). Briefly,
Rediject D-Luciferin Ultra (Perkin Elmer) was added in 0.2 ml fresh media
(1–200 dilution) to each well of cells in a 96-well plate and incubated for 15
min at 37°C. Luciferase activity was imaged with the IVIS Lumina II In Vivo
Imaging System (Perkin Elmer). The radiance of each well was determined using Living
Image 4.2 software (Perkin Elmer), background corrected by subtracting the mean
signal from empty wells and normalized both to the relative cell number of each well
as determined by Syto60 assay and the resulting normalized mean value of untreated
wells.
Rothenberg S.M., Concannon K., Cullen S., Boulay G., Turke A.B., Faber A.C., Lockerman E.L., Rivera M.N., Engelman J.A., Maheswaran S, & Haber D.A. (2015). Inhibition of mutant EGFR in lung cancer cells triggers SOX2-FOXO6-dependent survival pathways. eLife, 4, e06132.
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3

In Vivo Bioluminescence Imaging

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RediJect D-Luciferin Ultra was purchased from PerkinElmer (770505). Prior to in vivo imaging, mice were shaved in the region of interest depicted in the figure. Bioluminescence images of LL/2-Luc2 bearing mice were captured with IVIS Lumina III system 15 min after intraperitoneal injection of D-Luciferin (30 mg/kg)30 (link). The IVIS acquisition control panel was set to following conditions for imaging: Exposure time = auto, Binning = medium, F/Stop = 1, Emission Filter = open. The bioluminescence images were analyzed using Living Image software from PerkinElmer.
Wang K., Donnelly C.R., Jiang C., Liao Y., Luo X., Tao X., Bang S., McGinnis A., Lee M., Hilton M.J, & Ji R.R. (2021). STING suppresses bone cancer pain via immune and neuronal modulation. Nature Communications, 12, 4558.
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4

Bioluminescent Imaging of Mice

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Mice were anesthetized using isoflurane and injected intraperitoneally with RediJect D-Luciferin Ultra (PerkinElmer) (200μL, 30mg/mL). After 10 minutes mice were imaged using the IVIS Spectrum In Vivo Imaging System (PerkinElmer).
Song E., Mao T., Dong H., Boisserand L.S., Antila S., Bosenberg M., Alitalo K., Thomas J.L, & Iwasaki A. (2020). VEGF-C-driven lymphatic drainage enables brain tumor immunosurveillance. Nature, 577(7792), 689-694.
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5

Bioluminescent Imaging of STS26T-Luc Tumor Growth

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For detection of growth of STS26T-Luc cells, mice were anesthetized with 3% isofluorane and given i.p. injections of RediJect D-Luciferin Ultra (Perkin Elmer) ten minutes prior to imaging using the IVIS Spectrum in vivo imaging system (Caliper Life Sciences) as described (46 ). Image analysis was performed and total flux emission (photons/second) in the region of interest was determined using the Living Image Software for the IVIS Spectrum.
Semenova G., Stepanova D.S., Dubyk C., Handorf E., Deyev S.M., Lazar A.J, & Chernoff J. (2017). Targeting Group I p21-Activated Kinases to Control Malignant Peripheral Nerve Sheath Tumor Growth and Metastasis. Oncogene, 36(38), 5421-5431.
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6

Bioluminescent Imaging of Mice

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Mice were anesthetized using isoflurane and injected intraperitoneally with RediJect D-Luciferin Ultra (PerkinElmer) (200μL, 30mg/mL). After 10 minutes mice were imaged using the IVIS Spectrum In Vivo Imaging System (PerkinElmer).
Song E., Mao T., Dong H., Boisserand L.S., Antila S., Bosenberg M., Alitalo K., Thomas J.L, & Iwasaki A. (2020). VEGF-C-driven lymphatic drainage enables brain tumor immunosurveillance. Nature, 577(7792), 689-694.
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7

In Vivo Bioluminescence Imaging of T4 and T4-AAV Particle Biodistribution in Mice

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As previously described (10 (link)), 5 × 1010 and 2 × 1011 T4 or T4-AAV particles were intramuscularly injected into BALB/c mice. At 0.25, 0.5, 1, 2, 5, 10, 15, 30, and 60 days after administration, 30 μg of RediJect D-Luciferin Ultra (PerkinElmer, MA), a luciferase substrate dissolved in 0.9% saline, was injected intraperitoneally. After 5 min, the mice were lightly anesthetized with 2% isoflurane and placed on an IVIS 200 bioluminescence whole-body imaging workstation (Caliper). The bioluminescence emission signal was quantified using the camera control program, Living Image software, and displayed in physical units of surface radiance, photons per second per centimeter squared per steradian (photons s−1 cm−2 sr−1).
Zhu J., Tao P., Mahalingam M., Sha J., Kilgore P., Chopra A.K, & Rao V. (2019). A prokaryotic-eukaryotic hybrid viral vector for delivery of large cargos of genes and proteins into human cells. Science Advances, 5(8), eaax0064.
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8

Bioluminescent Imaging of STS26T-Luc Tumor Growth

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For detection of growth of STS26T-Luc cells, mice were anesthetized with 3% isofluorane and given i.p. injections of RediJect D-Luciferin Ultra (Perkin Elmer) ten minutes prior to imaging using the IVIS Spectrum in vivo imaging system (Caliper Life Sciences) as described (46 ). Image analysis was performed and total flux emission (photons/second) in the region of interest was determined using the Living Image Software for the IVIS Spectrum.
Semenova G., Stepanova D.S., Dubyk C., Handorf E., Deyev S.M., Lazar A.J, & Chernoff J. (2017). Targeting Group I p21-Activated Kinases to Control Malignant Peripheral Nerve Sheath Tumor Growth and Metastasis. Oncogene, 36(38), 5421-5431.
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9

Bioluminescent Imaging of Luciferase mRNA

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BALB/c mice were administered subcutaneously with 10 μg of luciferase mRNA loaded into LPP (LPP/Luc mRNA). Mice were injected intraperitoneally with 30 μg RediJect D-luciferin Ultra (Perkin-Elmer) 24 or 48 hours later, and bioluminescence was measured in a Xenogen IVIS-200 imaging system.
Persano S., Guevara M.L., Li Z., Mai J., Ferrari M., Pompa P.P, & Shen H. (2017). Lipopolyplex potentiates anti-tumor immunity of mRNA-based vaccination. Biomaterials, 125, 81-89.
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10

In Vivo Bioluminescence Imaging

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RediJect D-Luciferin Ultra was purchased from PerkinElmer (770505). Prior to in vivo imaging, mice were shaved in the region of interest depicted in the figure. Bioluminescence images of LL/2-Luc2 bearing mice were captured with IVIS Lumina III system 15 min after intraperitoneal injection of D-Luciferin (30 mg/kg) 27 (link) . The IVIS acquisition control panel was set to following conditions for imaging: Exposure time = auto, Binning = medium, F/Stop = 1, Emission Filter = open. The bioluminescence images were analyzed using Living Image software from PerkinElmer.
Wang K., Donnelly C.R., Jiang C., Liao Y., Tao X., Bang S., Lee M.K., Hilton M.J., & Ji R. (2021). STING suppresses cancer pain via immune and neuronal modulation.
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