Aflatoxins with standard mixture of AFB1 (1 mg/L), AFG1 (1 mg/L), AFB2 (0.3 mg/L), and AFG2 (0.3 mg/L) in MeOH, and Ochratoxin (OTA) standard (50 mg/L), glacial acetic acid, and formic acid were obtained from Sigma-Aldrich (Darmstadt, Germany). Ammonium formate and ammonium acetate were obtained from Agilent Technologies (Santa Clara, CA, USA). Sodium chloride and anhydrous magnesium sulfate, primary secondary amine (PSA), C18 sorbent, and LCMS-grade acetonitrile and methanol were purchased from Merck (Darmstadt, Germany). Ultra-pure water (ELGA) was used throughout this study.
Ammonium formate
Manufactured by Agilent Technologies
Sourced in United States, Italy, Germany
Ammonium formate is a chemical compound used in analytical laboratory applications. It is a salt formed by the combination of ammonium and formate ions. Ammonium formate is commonly used as a mobile phase additive in liquid chromatography-mass spectrometry (LC-MS) analysis to improve the ionization and separation of analytes.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Merck Group (4 mentions)
Methanol, by Merck Group (3 mentions)
Acetonitrile, by Merck Group (2 mentions)
Aflatoxins, by Merck Group (1 mentions)
Primary secondary amine, by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Tyrosine, by Merck Group (1 mentions)
Methanol, by Avantor (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Betulinic acid, by Merck Group (1 mentions)
Trypan blue reagent, by Merck Group (1 mentions)
Milli q integral water purification system, by Merck Group (1 mentions)
Phosphate buffer saline (pbs), by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Merck Group (1 mentions)
L glutamine, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium, by American Type Culture Collection (1 mentions)
Ham s f 12 medium, by American Type Culture Collection (1 mentions)
β glucuronidase, by Merck Group (1 mentions)
7 protocols using ammonium formate
1
Aflatoxin and Ochratoxin Quantification
2
Quantitative MDPA Analysis in Biological Matrices
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Water, acetonitrile, formic acid, ethyl acetate, methanol, and 3,4-Methylenedioxy-N-propylamphetamine (MDPA, internal standard, IS) were purchased from Sigma-Aldrich (Milan, Italy); ammonium formate, potassium dihydrogen phosphate, sodium hydroxide, and Bond Elute Certify 130 mg Solid Phase Extraction (SPE) columns were obtained from Agilent Technologies (Santa Clara, CA, United States).
3
AKB48 and JWH-209-D9 Quantification
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N-(1-adamantyl)-1-pentyl-1H-indazole-3-carboxamide (AKB48) and JWH-209-D9 were purchased from LGC Standards (Milan, Italy). Water, chloroform, formic acid, and methanol were purchased from 3V-Chemicals (Rome, Italy). Ammonium formate was purchased from Agilent (Agilent Technologies, Santa Clara, CA, United States). All reagents and solvents were of LC/MS grade.
4
Comprehensive Amino Acid Analysis
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Acetonitrile, methanol, and
water were purchased from Avantor (PA, USA), 99% formic acid (FA)
was purchased from Thermo Scientific (MA, USA), and ammonium formate
(AF) was purchased from Agilent (CA, USA), and all were LC-MS grade.
Hydrochloric acid (HCl), 88% FA, 30% hydrogen peroxide, barium hydroxide
(Ba(OH)2), sodium hydroxide, and phenol were acquired from
Thermo Scientific (MA, USA) and all of ACS grade. Reference AA phenylalanine
(Phe), leucine (Leu), isoleucine (Ile), valine (Val), proline (Pro),
tyrosine (Tyr), alanine (Ala), threonine (Thr), glycine (Gly), serine
(Ser), glutamic acid (Glu), aspartic acid (Asp), histidine (His),
arginine (Arg), lysine (Lys), methionine (Met), cystine (Cys2), tryptophan
(Trp), methionine sulfone (MetS), and cysteic acid (CysAc) were purchased
from Sigma-Aldrich (MO, USA). An AA standard mix was purchased from
Agilent (CA, USA), and a mix of U–13C,15N labeled canonical AA, used as an internal standard, was purchased
from Cambridge Isotope Laboratories (MA, USA).
water were purchased from Avantor (PA, USA), 99% formic acid (FA)
was purchased from Thermo Scientific (MA, USA), and ammonium formate
(AF) was purchased from Agilent (CA, USA), and all were LC-MS grade.
Hydrochloric acid (HCl), 88% FA, 30% hydrogen peroxide, barium hydroxide
(Ba(OH)2), sodium hydroxide, and phenol were acquired from
Thermo Scientific (MA, USA) and all of ACS grade. Reference AA phenylalanine
(Phe), leucine (Leu), isoleucine (Ile), valine (Val), proline (Pro),
tyrosine (Tyr), alanine (Ala), threonine (Thr), glycine (Gly), serine
(Ser), glutamic acid (Glu), aspartic acid (Asp), histidine (His),
arginine (Arg), lysine (Lys), methionine (Met), cystine (Cys2), tryptophan
(Trp), methionine sulfone (MetS), and cysteic acid (CysAc) were purchased
from Sigma-Aldrich (MO, USA). An AA standard mix was purchased from
Agilent (CA, USA), and a mix of U–13C,15N labeled canonical AA, used as an internal standard, was purchased
from Cambridge Isotope Laboratories (MA, USA).
5
Lipid-based nanoparticle formulation
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Cholesterol, L-α-phosphatidylcholine (lecithin), 1.2-distearoyl-sn-glycerol-3-phosphoethanolamine– N-[amino (polyethylene glycol)-2000] (DSPE-PEG2000), and betulinic acid were purchased from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany). Methanol was obtained from Merck (Darmstadt, Germany), ammonium formate from Agilent (Santa Clara, CA, USA), and ultrapure deionized water was provided by a Milli-Q® Integral Water Purification System from Merck Millipore (Darmstadt, Germany). All reagents were of analytical grade purity, commercially available. Specific cell culture media, high glucose Dulbecco’s Modified Eagle’s Medium (DMEM), Eagle’s Minimum Essential Medium (EMEM) and Dulbecco’s Modified Eagle’s Medium and Ham’s F12 medium (1:1 mixture) with L-Glutamine and HEPES (DMEM:F12) were purchased from ATCC (American Type Culture Collection). Fetal calf serum (heat-inactivated FCS), antibiotic mixture, Trypsin EDTA and Trypan blue reagent were purchased from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany). Epidermal Growth Factor (EGF) and phosphate buffer saline (PBS) were obtained from Thermo Fisher Scientific (Massachusetts, USA).
6
MTTA Metabolism Study Protocol
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MTTA standard (1 mg/mL) was from LGC, Milan, Italy. For the metabolism study, MTTA was from seized materials, characterized by different methods5 (link) to confirm its chemical identity and purified by high‐performance liquid chromatography (HPLC) before animal administration. Amphetamine D5 (1 mg/mL) was purchased from Chebios (Rome, Italy); water, acetonitrile, formic acid, methanol, and β‐glucuronidase were purchased from Sigma‐Aldrich (Milan, Italy); ammonium formate was from Agilent Technologies (Santa Clara, CA, USA).
Working solutions of MTTA and of the internal standard (IS) amphetamine D5 were prepared at a concentration of 1 μg/mL. Working solutions were stored at −20°C until use.
Working solutions of MTTA and of the internal standard (IS) amphetamine D5 were prepared at a concentration of 1 μg/mL. Working solutions were stored at −20°C until use.
7
Quantification of Nivalenol and Metabolites
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MeOH and ACN (both LC gradient grade), as well as glacial acetic acid (p.a.) were purchased from VWR International GmbH (Vienna, Austria) and water was purified using a Purelab Ultra system (ELGA, LabWater, Celle, Germany). Ammonium formate was obtained as a 5 M aqueous solution from Agilent Technologies (Waldbronn, Germany). Ammonium acetate, formic acid (both LC-MS grade), crystalline NIV-standards from Fusarium nivale (#N-7769; discontinued) and certified reference material IRMM-316 (NIV in ACN; certified value 24.0 ± 1.1 µg/g; indicative value: 18.8 ± 0.9 µg/mL) were obtained from Sigma Aldrich (Vienna, Austria). Standards for NIV (10.1 µg/mL) and FUSX (10.2 µg/mL) were purchased as a mixed solution of type A and B trichothecenes as well as zearalenone (#002002) in ACN from Romer Labs GmbH (Tulln, Austria). The same supplier also delivered a certified NIV calibrant (100 µg/mL) in ACN (#002011). Solid nivalenol hydrate (sc-236183) was obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA), weighted in using a Sartorius M500 P microbalance (Göttingen, Germany) and dissolved in MeOH to obtain a 1000 mg/L solution. PHS was previously purified and characterized by one- and two-dimensional nuclear magnetic resonance spectroscopy, as well as X-ray single-crystal diffraction [16 (link)].
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