Cells were maintained in the low- or high-glucose medium prior to experiments. A total of 2.0×103 cells per well were placed in 96-well plates in 100 μL of DMEM with the low or high glucose concentration. Cell viability was evaluated by a WST-8 assay using a cell count reagent kit (07553, Nacalai Tesque, Kyoto, Japan). The absorbance was measured at 450 nm using a microplate reader (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions.
Cell count reagent kit
Manufactured by Nacalai Tesque
Sourced in Japan
The Cell Count Reagent Kit is a laboratory tool designed to facilitate the enumeration of cells in a sample. It provides the necessary reagents and protocols to accurately determine the concentration of cells present in a given solution.
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Lab products found in correlation
2 protocols using cell count reagent kit
1
Cell Viability Evaluation using WST-8 Assay
2
Evaluating Primary Hepatocyte Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro, PHH viability was evaluated using WST‐8 assays with cell count reagent kit (07553; Nacalai Tesque, Kyoto, Japan). The absorbance was measured at 450 nm with VARIOSKAN LUX (Thermo Fisher Scientific) according to the manufacturer’s instructions. PHH viability was also evaluated by analyzing the confluency of PHHs with Incucyte S1 (Sartorius, Göttingen, Germany).
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In vitro, PHH viability was evaluated using WST‐8 assays with cell count reagent kit (07553; Nacalai Tesque, Kyoto, Japan). The absorbance was measured at 450 nm with VARIOSKAN LUX (Thermo Fisher Scientific) according to the manufacturer’s instructions. PHH viability was also evaluated by analyzing the confluency of PHHs with Incucyte S1 (Sartorius, Göttingen, Germany).
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