Timstof flex maldi qtof mass spectrometer

Manufactured by Bruker

Sourced in Germany

The TimsTOF fleX MALDI-QTOF mass spectrometer is a high-performance instrument designed for sensitive and accurate mass analysis. It combines a MALDI ion source with a Trapped Ion Mobility Spectrometry (TIMS) analyzer and a quadrupole time-of-flight (QTOF) mass analyzer. The core function of this instrument is to provide high-resolution mass spectrometry data for a variety of analytical applications.

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Lab products found in correlation

Smartbeam 3d laser, by Bruker (1 mentions)

5 protocols using timstof flex maldi qtof mass spectrometer

MALDI-QTOF Imaging of Biological Samples

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A dual source timsTOF fleX MALDI-QTOF mass spectrometer (Bruker) was used to image the slides as previously described (McDowell et al., 2021 (link)). Images were collected with a SmartBeam 3D laser operating at 10,000 Hz with a 20 µm laser spot size at a 150 µm raster with 300 laser shots per pixel. Samples were analyzed in positive ion mode spanning a m/z range of 700–4,000.

Blaschke C.R., Hartig J.P., Grimsley G., Liu L., Semmes O.J., Wu J.D., Ippolito J.E., Hughes-Halbert C., Nyalwidhe J.O, & Drake R.R. (2021). Direct N-Glycosylation Profiling of Urine and Prostatic Fluid Glycoproteins and Extracellular Vesicles. Frontiers in Chemistry, 9, 734280.

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MALDI-QTOF Imaging Mass Spectrometry

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The slides were imaged with a timsTOF fleX MALDI-QTOF mass spectrometer (Bruker, Billerica, MA) with a SmartBeam 3D laser operating at 10,000 Hz and 20 µm laser spot size. There were 300 laser shots collected per pixel, and a 150 µm raster was used. A 700–4000 m/z range was scanned in positive ion mode.

Blaschke C.R., Hill E.G., Mehta A.S., Angel P.M., Laronga C, & Drake R.R. (2022). Integrating age, BMI, and serum N-glycans detected by MALDI mass spectrometry to classify suspicious mammogram findings as benign lesions or breast cancer. Scientific Reports, 12, 20801.

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MALDI-qTOF Mass Spectrometry Imaging

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Mass spectrometry imaging was performed using a Bruker timsTOF fleX MALDI qTOF mass spectrometer (Bruker Daltonics, Bremen, Germany) operated in either negative ion mode or positive ion mode with TIMS off. Data were collected from m/z 50 to m/z 1000, with tune parameters optimized for sensitivity of analytes < m/z 300 as follow: Funnel 1 RF of 50 Vpp; Funnel 2 RF of 100 Vpp; Multipole RF of 150 Vpp; Collision RF of 600 Vpp; Transfer Time of 35 μs; and Pre Pulse Storage of 2 μs. Images were collected with 100 μm spatial resolution with a beam scan of 96 μm. A total of 1200 laser shots were summed per pixel with the laser operating at 10 kHz. Data were visualized using SCiLS Lab 2021b (Version 9.01.12514).

Sowers J.L., Sowers M.L., Shavkunov A.S., Hawkins B.E., Wu P., DeWitt D.S., Prough D.S, & Zhang K. (2021). Traumatic brain injury induces region-specific glutamate metabolism changes as measured by multiple mass spectrometry methods. iScience, 24(10), 103108.

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High-Resolution N-Glycan Imaging

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Tissue samples were analyzed using a dual-source timsTOFfleX MALDI-QTOF mass spectrometer (Bruker) operating in positive mode. A 10 kHz SmartBeam 3D laser with a 20 µm laser spot size was run at a 40 µm raster with 300 laser shots per pixel to produce high-resolution images of N-glycan spatial localization in the m/z range of 700–4,000.

Young L.E., Nietert P.J., Stubler R., Kittrell C.G., Grimsley G., Lewin D.N., Mehta A.S., Hajar C., Wang K., O’Quinn E.C., Angel P.M., Wallace K, & Drake R.R. (2024). Utilizing multimodal mass spectrometry imaging for profiling immune cell composition and N-glycosylation across colorectal carcinoma disease progression. Frontiers in Pharmacology, 14, 1337319.

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MALDI-QTOF Imaging of Tissues and Serum

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All tissues and serum samples were imaged using a timsTOF Flex MALDI-QTOF mass spectrometer (Bruker Daltonics); (m/z 500–4000) operating in a positive mode. Focus Pre TOF parameters were set as followed: transfer time 120.0 μs and pre-pulse storage 25.0 μs. For whole tissues (normal, HCC, and iCCA) and TMAs images were collected at 100 μm raster. For serum samples, images were collected at 150 μm raster. All images were collected at 200 laser shots per pixel.

Ochoa-Rios S., Blaschke C.R., Wang M., Peterson K.D., DelaCourt A., Grauzam S.E., Lewin D., Angel P., Roberts L.R., Drake R, & Mehta A.S. (2023). Analysis of N-linked Glycan Alterations in Tissue and Serum Reveals Promising Biomarkers for Intrahepatic Cholangiocarcinoma. Cancer Research Communications, 3(3), 383-394.

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