Acid-terminated poly(D,L-lactide-co-glycolide) (PLGA), of low inherent viscosity (low molecular weight; PLGALo) in hexafluoro-2-propanol ~ 0.17 dL/g (approx. 4,200 g/mol) and high inherent viscosity (high molecular weight; PLGAHi) in hexafluoro-2-propanol ~ 0.66 dL/g (approx. 43,500 g/mol) monomer ratios 50:50 and acid terminated poly(D, L-lactide) (PLA) of low inherent viscosity (low molecular weight) in hexafluoro-2-propanol ~ 0.21 dL/g (approx. 11,700 g/mol) were purchased from Lactel Absorbable Polymers (Birmingham, AL). Poly(ethylene-alt-maleic anhydride) (PEMA) was purchased from Polysciences, Inc. (Warrington, PA). Poly(vinyl alcohol) (PVA, MW 30,000-70,000), β-mercaptoethanol, and LPS from Escherichia Coli serotype O111:B4 were obtained from Sigma-Aldrich (St. Louis, MO). CpG-ODN 1668 was obtained from Invivogen (San Diego, CA).
Poly ethylene alt maleic anhydride pema
Manufactured by Polysciences
Poly(ethylene-alt-maleic anhydride) (PEMA) is a copolymer composed of ethylene and maleic anhydride units. It is a white to off-white powder. PEMA is used as a raw material in various applications, including paints, coatings, and adhesives.
Automatically generated - may contain errors
Lab products found in correlation
Lps from escherichia coli serotype o111 b4, by Merck Group (3 mentions)
Proteolipid peptide nh2 plp139 15, by GenScript (2 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Cpg odn 1668, by InvivoGen (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Amine terminated ovalbumin peptide nh2 ova323 339, by GenScript (1 mentions)
Fetal bovine serum (fbs), by Avantor (1 mentions)
Versene, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Peanut extract, by Stallergenes Greer (1 mentions)
Aluminum hydroxide alum alhydrogel, by InvivoGen (1 mentions)
Cyanine 5.5 amine, by Lumiprobe (1 mentions)
6 protocols using poly ethylene alt maleic anhydride pema
1
Polymers for Immunomodulatory Biomaterials
2
Polymer-Peptide Conjugate Synthesis
3
Polymeric Biomaterials Synthesis and Characterization
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Acid‐terminated poly(D,L‐lactide) (PLA) of low inherent viscosity in hexafluoro‐2‐propanol ~0.21 dL/g (approximate MW 11,300 g/mol) and acid‐terminated poly(D,L‐lactide‐co‐glycolide) (PLGA), of low inherent viscosity in hexafuoro‐2‐propanol ~0.17 dL/g (approximate MW 4200 g/mol) were purchased from Lactel Absorbable Polymers located at Birmingham, AL. Poly(ethylene‐alt‐maleic anhydride) (PEMA) (MW 400,000 g/mol) was purchased from Polysciences, Inc. located at Warrington, PA. LPS (Escherichia coli O111:B4, #L2630) and RPMI 1640 (supplemented with L‐glutamine, #R8758) were obtained from Millipore Sigma (St. Louis, MO). Penicillin–streptomycin, Versene, and NuPAGE™ 12% Bis‐Tris 1.0 mm Mini Protein Gel (#NPO343BOX) were purchased from Thermo Fisher Scientific (Waltham, MA). Fetal bovine serum (FBS, #97068‐085) was purchased from VWR (Radnor, PA). L929 fibroblast cells (NCTC clone 929) were purchased from the American Type Culture Collection (ATCC, Manassas, VA). 2× SDS/PAGE sample buffer was produced using 4% SDS, 5.7 M β‐mercaptoethanol, 0.2 M Tris/HCl, pH 6.8, 20% glycerol, and 5 mM EDTA. All chemicals were of analytical grade and obtained from Millipore Sigma located in St. Louis, MO, and CombiBlocks located in San Diego, CA.
4
Functionalization of PLGA for Bioconjugation
5
Peanut Allergy Immunization and Sensitization
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Peanut extract (Greer Laboratories, Lenoir, NC) was used for all intraperitoneal (i.p.) and intravenous (i.v.) immunizations and in NP formulations. For delayed type hypersensitivity (DTH) studies and for intragastric sensitization and challenge, peanut flour (Byrd Mill 12% fat, light roast) was solubilized in PBS and concentrated using a 10,000 MWCO Centricon filter unit to create a Peanut extract. Aluminum hydroxide (alum; alhydrogel) was purchased from InvivoGen (San Diego, CA). Cholera toxin (CTx) from Vibrio cholera was purchased from List Biological Laboratories (Campbell, CA). PLG (50:50) with a single carboxylic acid end group and an inherent viscosity of 0.17 dL/g in hexafluoro-2-propanol was purchased from Lactel Absorbable Polymers (Essen, Germany). Poly(ethylene-alt-maleic anhydride) (PEMA) was purchased from Polyscience (Niles, IL). Grade V hen egg albumin (Ovalbumin, OVA) was purchased from Sigma Aldrich (St. Louis, MO). Cyanine5.5 amine was purchased from Lumiprobe (Cockeysville, MD). Chromogenic LAL endotoxin quantitation kit was purchased from ThermoFisher Scientific (Waltham, MA). All other reagents were purchased from Sigma Aldrich unless noted otherwise.
6
Polymer-Peptide Conjugate Synthesis
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Acid‐terminated 50:50 poly(d ,l ‐lactide‐co‐glycolide) (PLG) (∼0.17 dl/g inherent viscosity in hexafluoro‐2‐propanol) was purchased from Lactel Absorbable Polymers. Poly(ethylene‐alt‐maleic anhydride) (PEMA) was purchased from Polysciences, Inc. Amine‐terminated ovalbumin peptide (NH2‐OVA323‐339) (OVA), and proteolipid peptide (NH2‐PLP139‐15) (PLP), were purchased from Genscript. LPS from Escherichia coli serotype O111:B4 was purchased from Sigma‐Aldrich. N‐(3‐Dimethylaminopropyl)‐N′‐ethylcarbodiimide hydrochloride (ECDI) was purchased from Millipore.
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