Hiseq v4 paired end flow cell

Manufactured by Illumina

The HiSeq v4 paired end flow cell is a consumable product used in Illumina's HiSeq DNA sequencing instruments. It provides the physical substrate for DNA samples to be analyzed, enabling the generation of high-quality, paired-end sequencing data.

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Lab products found in correlation

Hiseq pe cluster kit v4 cbot, by Illumina (2 mentions) Hiseq sbs kit v4 sequencing, by Illumina (1 mentions) Hiseq 2500, by Illumina (1 mentions) Hiseq sbs kit v4 sequencing reagents, by Illumina (1 mentions) Hiseq instrument, by Illumina (1 mentions) Truseq stranded mrna sample preparation kit, by Illumina (1 mentions) Rneasy kit, by Qiagen (1 mentions)

2 protocols using hiseq v4 paired end flow cell

Illumina HiSeq 2500 Paired-End Sequencing

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Sequencing libraries (25 pM) were chemically denatured and applied to an Illumina HiSeq v4 paired end flow cell using an Illumina cBot. Hybridized molecules were clonally amplified and annealed to sequencing primers with reagents from an Illumina HiSeq PE Cluster Kit v4-cBot. Following transfer of the flow cell to an Illumina HiSeq 2500 instrument (HCS v2.2.38 and RTA v1.18.61), a 125 cycle paired-end sequence run was performed using HiSeq SBS Kit v4 sequencing reagents.

Wolff R.K., Hoffman M.D., Wolff E.C., Herrick J.S., Sakoda L.C., Samowitz W.S, & Slattery M.L. (2018). Mutation analysis of adenomas and carcinomas of the colon: Early and late drivers. Genes, Chromosomes & Cancer, 57(7), 366-376.

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RNA-seq Library Preparation and Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was extracted from cells using RNeasy Kit from Qiagen. The RNA-seq libraries were generated using Illumina TruSeq Stranded mRNA sample preparation kit with oligo dT selection. Sequencing libraries (25 pM) were chemically denatured and applied to an Illumina HiSeq v4 paired end flow cell using an Illumina cBot. Hybridized molecules were clonally amplified and annealed to sequencing primers with reagents from an Illumina HiSeq PE Cluster Kit v4-cBot. Following transfer of the flowcell to an Illumina HiSeq instrument at the University of Utah High-Throughput Genomics Core, a 125 cycle paired-end sequence run was performed using HiSeq SBS Kit v4 sequencing reagents with six samples multiplexed in each Illumina lane. The average number of reads per sample ranged from 38 to 58 million reads.

Wagner A.H., Devarakonda S., Skidmore Z.L., Krysiak K., Ramu A., Trani L., Kunisaki J., Masood A., Waqar S.N., Spies N.C., Morgensztern D., Waligorski J., Ponce J., Fulton R.S., Maggi LB J.r., Weber J.D., Watson M.A., O’Conor C.J., Ritter J.H., Olsen R.R., Cheng H., Mukhopadhyay A., Can I., Cessna M.H., Oliver T.G., Mardis E.R., Wilson R.K., Griffith M., Griffith O.L, & Govindan R. (2018). Recurrent WNT pathway alterations are frequent in relapsed small cell lung cancer. Nature Communications, 9, 3787.

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