The slope of the line acquired from the plot of integrated fluorescence intensity versus absorbance is represented by (m) and ƞ represents the refractive index of the solvent used [56 ]. The R subscript denotes the reference fluorophore with a known quantum yield. The fluorescence spectra were measured by exciting the samples at 465 nm, collecting readings from 490 to 850 nm, baseline corrected, integrated, and plotted using SigmaPlot® Software. The 465 nm excitation wavelength was chosen in order to align with the excitation parameters of our institution’s In Vivo Imaging System (IVIS).
Du 730 life sciences uv vis spectrophotometer
The DU 730 Life Sciences UV/Vis spectrophotometer is a laboratory instrument that measures the absorption of ultraviolet (UV) and visible light by a sample. It is designed to analyze the chemical and biological properties of a wide range of materials.
Lab products found in correlation
3 protocols using du 730 life sciences uv vis spectrophotometer
Optical Absorption and Fluorescence Characterization of Nanoparticles
Protein Purification and Analysis
Protein Purification and Analysis
During protein purification, proteolysis was inhibited by adding Pierce™ Protease Inhibitor tablets (EDTA free; Thermo Scientific) to lysis buffer. Affinity chromatography and SDS-PAGE analysis were conducted using HisPur™ Nickel-nitrilotriacetic acid-agarose (Ni-NTA) resin (Thermo) and 10% or 4–15% Mini-PROTEAN TGX Precast SDS-PAGE gels (Bio-Rad) with 2× Laemmli sample buffer (Bio-Rad). Protein concentrations were determined by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo).
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