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Freezeview software

Manufactured by Actimetrics
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FreezeView is a software suite designed for the analysis and visualization of cryogenic electron microscopy (cryo-EM) data. The software provides tools for processing, analyzing, and interpreting cryo-EM images and molecular structures. It offers features for particle picking, 2D and 3D image processing, and model building. FreezeView is an essential tool for researchers working with cryo-EM technology.

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Lab products found in correlation

Motor monitor software, by Kinder Scientific (4 mentions) Freezeframe software, by Actimetrics (1 mentions)

9 protocols using freezeview software

1

Automated and Blinded Behavioral Scoring

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During testing, investigators were not blind to condition. However, results were replicated across several cohorts. Videos for behavioral scoring (i.e., freezing behavior) were analyzed using FreezeView softwares (Actimetrics, Wilmette, IL) during sessions without light application. For sessions in which mice received photostimulation (silencing), the light coming out of the implants prevented automatic scoring and was therefore independently scored by 2 investigators. Other analyses of behavior such as ambulation in the open-field was scored automatically using MotorMonitor Software (Kinder Scientific, Poway, CA). Anxiety assessment in the elevated plus maze and novelty suppressed feeding tests were carried out by 1 investigator blinded to treatment and/or genotype. Sahay (PI) selected individuals in the lab to perform independent scoring.
Besnard A., Gao Y., Kim M.T., Twarkowski H., Reed A.K., Langberg T., Feng W., Xu X., Saur D., Zweifel L.S., Davison I, & Sahay A. (2019). Dorsolateral septum somatostatin interneurons gate mobility to calibrate context specific behavioral fear responses. Nature neuroscience, 22(3), 436-446.
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2

Automated and Blinded Behavioral Scoring

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During testing, investigators were not blind to condition. However, results were replicated across several cohorts. Videos for behavioral scoring (i.e., freezing behavior) were analyzed using FreezeView softwares (Actimetrics, Wilmette, IL) during sessions without light application. For sessions in which mice received photostimulation (silencing), the light coming out of the implants prevented automatic scoring and was therefore independently scored by 2 investigators. Other analyses of behavior such as ambulation in the open-field was scored automatically using MotorMonitor Software (Kinder Scientific, Poway, CA). Anxiety assessment in the elevated plus maze and novelty suppressed feeding tests were carried out by 1 investigator blinded to treatment and/or genotype. Sahay (PI) selected individuals in the lab to perform independent scoring.
Besnard A., Gao Y., Kim M.T., Twarkowski H., Reed A.K., Langberg T., Feng W., Xu X., Saur D., Zweifel L.S., Davison I, & Sahay A. (2019). Dorsolateral septum somatostatin interneurons gate mobility to calibrate context specific behavioral fear responses. Nature neuroscience, 22(3), 436-446.
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3

Behavioral Scoring in Optogenetic Studies

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During testing, investigators were not blind to conditions. However, results were replicated across several cohorts. Videos for behavioral scoring (i.e., freezing behavior) were analyzed using FreezeView softwares (Actimetrics, Wilmette, IL) during sessions without light application. For sessions in which mice received photostimulation (or silencing), the light coming out of the implants prevented automatic scoring and was therefore independently scored by 2 investigators. Other analyses of behavior such as ambulation in the open-field was scored automatically using MotorMonitor Software (Kinder Scientific, Poway, CA). Anxiety assessment in the elevated plus maze and novelty suppressed feeding tests were carried out by 1 investigator blinded to treatment and/or genotype. Sahay (PI) selected individuals in the lab to perform independent scoring.
Besnard A., Miller S.M, & Sahay A. (2020). Distinct Dorsal and Ventral Hippocampal CA3 Outputs Govern Contextual Fear Discrimination. Cell reports, 30(7), 2360-2373.e5.
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4

Behavioral Scoring in Optogenetic Studies

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During testing, investigators were not blind to conditions. However, results were replicated across several cohorts. Videos for behavioral scoring (i.e., freezing behavior) were analyzed using FreezeView softwares (Actimetrics, Wilmette, IL) during sessions without light application. For sessions in which mice received photostimulation (or silencing), the light coming out of the implants prevented automatic scoring and was therefore independently scored by 2 investigators. Other analyses of behavior such as ambulation in the open-field was scored automatically using MotorMonitor Software (Kinder Scientific, Poway, CA). Anxiety assessment in the elevated plus maze and novelty suppressed feeding tests were carried out by 1 investigator blinded to treatment and/or genotype. Sahay (PI) selected individuals in the lab to perform independent scoring.
Besnard A., Miller S.M, & Sahay A. (2020). Distinct Dorsal and Ventral Hippocampal CA3 Outputs Govern Contextual Fear Discrimination. Cell reports, 30(7), 2360-2373.e5.
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5

Freezing Behavior Analysis Protocol

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During testing, investigators were not blind to conditions. However, freezing behavior was analyzed using FreezeView softwares (Actimetrics, Wilmette, IL).
Besnard A, & Sahay A. (2020). Enhancing adult neurogenesis promotes contextual fear memory discrimination and activation of hippocampal-dorsolateral septal circuits. Behavioural brain research, 399, 112917.
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6

Fear Conditioning Protocol for Memory Assessment

Cited 2 times
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Fear conditioning was performed as previously described with some modifications (Huang et al., 2013 (link); Zhu et al., 2011 (link)). Briefly, mice were first handled for 5 min for two consecutive days. On the training day, after 2 min in the conditioning chamber, mice received two pairings of a tone (2,800 Hz, 85 dB, 30 s) with a co-terminating foot shock (0.7 mA, 2 s), after which they remained in the chamber for an additional minute and were then returned to their home cages. 24 h after training, mice were tested for freezing (immobility except for respiration) in response to the training context (training chamber). Freezing behavior was automatically scored by FreezeView software (Actimetrics). The percentage of time spent freezing was taken as an index of learning and memory.
Johnson J.L., Stoica L., Liu Y., Zhu P.J., Bhattacharya A., Buffington S.A., Huq R., Eissa N.T., Larsson O., Porse B.T., Domingo D., Nawaz U., Carroll R., Jolly L., Scerri T.S., Kim H.G., Brignell A., Coleman M.J., Braden R., Kini U., Jackson V., Baxter A., Bahlo M., Scheffer I.E., Amor D.J., Hildebrand M.S., Bonnen P.E., Beeton C., Gecz J., Morgan A.T, & Costa-Mattioli M. (2019). Inhibition of Upf2-Dependent Nonsense-Mediated Decay Leads to Behavioral and Neurophysiological Abnormalities by Activating the Immune Response. Neuron, 104(4), 665-679.e8.
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7

Fear Conditioning in APP/PS1 Mice

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APPswe/PS1∆E9 mice treated with DMSO, AcA, or TTF were placed into a 17.8 × 17.8 × 30.5 cm chamber with two clear Plexiglass walls, two metal walls, and a stainless-steel grid floor (Coulbourn Instruments). The chamber was located inside an opaque isolation cabinet. The mice remained in the cage for 148 s before receiving a single 0.75 mA foot shock lasting 2 s. The mice remained in the cage for another 30 s after the shock before being returned to their home cage. Twenty-four hours later the mice were returned to the chamber for 5 min. Freezing was measured using a digital video camera mounted above the chamber and FreezeFrame software (Actimetrics). Percentage freezing time was analyzed using FreezeView software (Actimetrics).
Bartolotti N., Disouky A., Kalinski A., Elmann A, & Lazarov O. (2018). Phytochemicals from Achillea fragrantissima are Modulators of AβPP Metabolism. Journal of Alzheimer's disease : JAD, 66(4), 1425-1435.
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8

Auditory Fear Memory Consolidation

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One day after training, rats received 20 presentations of the tones (30 s each) without any footshocks in context A. The intertone interval was of 60 s. Rats remained in the boxes for 60 s after the last tone presentation.
Digital cameras recorded the animals' behavior, and memory was evaluated by a blind experimenter measuring the time spent freezing during the tone presentation, using Freeze View software (Actimetrics). Freezing was defined as immobilization except for respiration.
Haubrich J., Bernabo M, & Nader K. (2020). Noradrenergic projections from the locus coeruleus to the amygdala constrain fear memory reconsolidation. eLife, 9, e57010.
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9

Contextual Fear Memory Assessment

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Contextual fear conditioning consisted of a 2-min period of chamber acclimatation, followed by two presentations of a 2-s foot shock of 0.6 mA separated by 1 min. The mice were returned to their home cage 1 min after the last shock. After training, on day 2 and 3, the mice were placed again in the conditioning chamber for an extinction trial which consisted in placing the mice in the chamber for 15 min without any foot shock. On day 4, the mice were tested for contextual fear memory by placing them in the conditioning chamber for a 5-min period. For all tests, behavior was recorded and analysed using FreezeView software (Actimetrics, USA). Freezing, the conditioned response indicating fear memory retention, was analyzed for each mouse at in 3 min interval for extinction sessions and as a 5-min trial for the test sesión. Data are expressed as the percentage of time freezing in each interval.
Gibon J., Unsain N., Gamache K., Thomas R.A., De Leon A., Johnstone A., Nader K., Séguéla P, & Barker P.A. (2016). The X-linked inhibitor of apoptosis regulates long-term depression and learning rate. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30(9).
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