Small RNA was isolated from 30 mg gWAT and 5 mg liver using the PureLink miRNA Kit (Invitrogen) according to the manufacturer's protocol. miR-31 (000185, Thermo Fisher Scientific, Germany), miR-31* (002495, Thermo Fisher Scientific), and snoRNA202 (001232, Thermo Fisher Scientific) were reverse transcribed individually using 30 ng for gWAT and 150 ng miRNA for liver with the TaqMan miRNA Reverse Transcription Kit according to vendor's instructions (Thermo Fisher Scientific). All qPCRs were performed with the Roche LightCycler® 480/384 (Roche, Switzerland). Relative expression of miRNA levels was evaluated using snoRNA202 as endogenous control.
Mir 31
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The MiR-31 is a laboratory instrument designed to quantify the expression levels of the microRNA-31 (miR-31) in biological samples. It utilizes real-time PCR technology to detect and measure the abundance of this specific microRNA. The core function of the MiR-31 is to provide accurate and reliable data on miR-31 expression, which can be used in various research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Mir 130a, by Thermo Fisher Scientific (2 mentions)
Purelink mirna kit, by Thermo Fisher Scientific (1 mentions)
Taqman mirna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Snorna202, by Thermo Fisher Scientific (1 mentions)
Lightcycler 480 384, by Roche (1 mentions)
7500 software v2, by Thermo Fisher Scientific (1 mentions)
Mir 204 5p, by Thermo Fisher Scientific (1 mentions)
3 protocols using mir 31
1
Quantifying miRNA Levels in gWAT and Liver
2
Quantification of miRNA Expression
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Taqman RT and stem–loop real-time assay were from Applied Biosystems: miR-31 (assayID: 002279), miR-130a (assayID: 000454). Briefly, 100 ng RNAs were reverse transcribed by specific stem–loop primer and further analyzed by Taqman real-time PCR assay using default setting. U6 snRNA (assayID: 001973) was used as an internal loading control. Data were analyzed by Applied Biosystems 7500 software V2.0.1.
3
Quantitative Real-Time PCR Assay for miRNA Expression
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Taqman RT and stem-loop real-time assay were from Applied Biosystems: miR-31 (assayID: 002279), miR-130a (assayID: 000454), miR-204-5p (assayID: 000508) and miR-1236 (assayID: 002761). Briefly, 100 ng RNAs were reverse transcribed by specific stem-loop primer and further analyzed by Taqman real-time PCR assay using default setting. U6 snRNA (assayID: 001973) was used as an internal loading control. Data were analyzed by Applied Biosystems 7500 software V2.0.1. MiR-204 hairpin precursor can generate both miR-204-5p (miR-204) and its complementary miR-204-3p (miR-204*) from the other arm. We focused on miR-204-5p here because the LNA experiment (Fig. 2D–F ) confirmed the anti-HBV potential from miR-204-5p. In this report, we used the old name miR-204 and the new name miR-204-5p interchangeably.
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