Thermo q exactive hybrid quadrupole orbitrap mass spectrometer

Manufactured by Thermo Fisher Scientific

The Thermo Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer is a high-resolution, accurate-mass (HRAM) mass spectrometer that combines a quadrupole mass filter and an Orbitrap mass analyzer. It is designed to provide high-performance MS and MS/MS capabilities for a wide range of analytical applications.

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Lab products found in correlation

Ultimate 3000 hplc, by Thermo Fisher Scientific (1 mentions) Sciex qtrap 5500, by AB Sciex (1 mentions) Serum triglyceride determination kit, by Merck Group (1 mentions)

Close spelling variants of this product

Thermo Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer Thermo quadrupole-orbitrap Q-Exactive Mass Spectrometer Q Exactive hybrid Q–Orbitrap mass spectrometer Q Exactive orbitrap hybrid mass spectrometer Q Exactive Hybrid Quadrupole Mass Spectrometer Q-Exactive quadrupole-Orbitrap mass spectrometer Q Exactive Hybrid Quadrupole-Orbitrap Thermo Q Exactive™ Orbitrap™ mass Hybrid quadrupole orbitrap mass spectrometer

2 protocols using thermo q exactive hybrid quadrupole orbitrap mass spectrometer

LC-FTMS Metabolomics Data Analysis

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Samples were analyzed by LC-FTMS, as previously described [3 (link)]. m/z features were collected by a Thermo Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher, San Diego, CA) from m/z 85 to 1275 over 10 min. Peak extraction, noise removal, and quantification of ion intensities were performed by an adaptive processing software package (apLCMS) with xMSanalyzer designed for use with LC-FTMS data [34 (link)]. For clarification, data extracted on m/z features, which are not definitively identified metabolites, will be referred to as “features” and not metabolites. See Additional file 2.

Cribbs S.K., Uppal K., Li S., Jones D.P., Huang L., Tipton L., Fitch A., Greenblatt R.M., Kingsley L., Guidot D.M., Ghedin E, & Morris A. (2016). Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection. Microbiome, 4, 3.

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Liver Metabolite Analysis by LC-MS

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Liver samples were minced and extracted in 0.4N perchloric acid. The 1C cycle metabolites in the liver homogenates were detected by liquid chromatography–mass spectrometry (LC–MS) analysis on a Sciex 5500 QTrap with Agilent 1200 LC (AB Sciex.) for SAM and SAH, and a Thermo Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer with an Ultimate 3000 HPLC (Thermo Fisher Scientific) for PE and PC. Liver TG levels were measured using a Serum Triglyceride Determination Kit (TR0100, Sigma-Aldrich).

Kim Y.C., Seok S., Byun S., Kong B., Zhang Y., Guo G., Xie W., Ma J., Kemper B, & Kemper J.K. (2018). AhR and SHP regulate phosphatidylcholine and S-adenosylmethionine levels in the one-carbon cycle. Nature Communications, 9, 540.

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