C kit pe

Manufactured by Thermo Fisher Scientific

Sourced in United States

The C-Kit-PE is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is a specialized tool used in various research and analytical applications. The core function of the C-Kit-PE is to detect and analyze the expression of the c-Kit protein, which is a receptor tyrosine kinase that plays a crucial role in cellular processes. The product utilizes a fluorescent phycoerythrin (PE) label to facilitate the detection and quantification of c-Kit expression levels in samples.

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Lab products found in correlation

Close spelling variants of this product

C-Kit-PE-CY7 (4 citations) Kit c (2 citations) PE conjugated anti-mouse Sca1 and APC conjugated anti-mouse c-Kit (2 citations) PE-conjugated rat anti-mouse c-kit antibody (2 citations) PE-conjugated anti-mouse c-kit antibody (2 citations)

5 protocols using c kit pe

Hematopoietic Lineage Analysis in Knockout Mice

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For analysis of hematopoietic lineage markers in the BMs of mice with specific knockout of Wls or Six1 in hematopoietic cells, we follow protocols described previously [30 (link)]. Characterization of MLL-AF9 AML and isolation of the enriched LIC population from BMs of AML mice have been described previously [28 (link)]. Fluorescein-labeled antibodies used in the study include: Biotin Mouse Lineage Depletion Cocktail (BD Biosciences); Streptavidin-PerCP-Cy5.5, Sca1-FITC, cKit-APC-Cy7, cKit-PE, FcγR-PE-Cy7, CD34-PacificBlue, FLT3-PE, IL7R-APC, Mac1-APC, B220-PE, CD3-APC, Gr1-PE, Mac1-APC, CD16/CD32, all antibodies are anti-mouse and were purchased from eBiosciences.

Zhang L.S., Kang X., Lu J., Zhang Y., Wu X., Wu G., Zheng J., Tuladhar R., Shi H., Wang Q., Morlock L., Yao H., Huang L.J., Maire P., Kim J., Williams N., Xu J., Chen C., Zhang C.C, & Lum L. (2018). Installation of a cancer promoting WNT/SIX1 signaling axis by the oncofusion protein MLL-AF9. EBioMedicine, 39, 145-158.

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Imaging Flow Cytometry of Erythroblasts

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Extensively self-renewing erythroblasts or β-yac ESREs were stained with CD71-FITC (eBioscience), c-Kit-PE (eBioscience), DAPI (Sigma-Aldrich, St Louis, MO, USA), and DRAQ5 (eBioscience) and run on the ImageStream (Amnis, Seattle, WA, USA). The data was analyzed with IDEAS software (Amnis) as previously published [24 (link)].

Getman M., England S.J., Malik J., Peterson K., Palis J, & Steiner L.A. (2014). Extensively self-renewing erythroblasts derived from transgenic β-yac mice is a novel model system for studying globin switching and erythroid maturation. Experimental hematology, 42(7), 536-46.e8.

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Dissociation and Intracellular Staining of Cardiac Cells

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Cells were dissociated using collagenase B (1 mg ml⁻¹, Roche) for 5–45 min at 37 °C (ref. 2 (link)). For staining of cardiac markers, 1.5 × 10⁵ cells were fixed and permeabilized according to the manufacturer's instructions (Fix & Perm; An der Grub). For other intracellular staining, cells were fixed and permeabilized by incubation in cold methanol (90%) for 15 min. Anti-cardiac Troponin T (1:200, clone 13-11, Thermo Scientific), anti-sarcomeric α-actinin (1:800, EA53, Sigma-Aldrich), anti-myosin heavy chain α (1:20, MF20, Hybridoma Bank), anti-T-brachyury (1:50, N-19, Santa Cruz), anti-vimentin, anti-CDX2, anti-TRA-1-60 (all 1:100, EPR3776, AMT28, TRA-1-60, respectively, Abcam), SSEA3, SSEA4 (1:100, MC-631 and MC-813-70, respectively, Hybridoma Bank) and respective isotype controls (DAKO) were detected using appropriate Cy3- or Cy5-conjugated antibodies (1:200; Jackson ImmunoResearch). EpCAM-FITC (1:100; EBA-1), NCAM-PE-CF594 (1:50; B159, both BD biosciences), CD90-APC (1:100, 5E10, Life Technologies), cKIT-PE and CXCR4-APC (1:100; both eBioscience) were incubated for 30 min at 4 °C. Data were acquired on an Accuri C6 flow cytometer (BD Biosciences) and analysed using FlowJo software (Treestar, Ashland, USA).

Kempf H., Olmer R., Haase A., Franke A., Bolesani E., Schwanke K., Robles-Diaz D., Coffee M., Göhring G., Dräger G., Pötz O., Joos T., Martinez-Hackert E., Haverich A., Buettner F.F., Martin U, & Zweigerdt R. (2016). Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells. Nature Communications, 7, 13602.

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Enrichment of Primary Hepatocytes

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Enrichment for primary hepatocytes (PH) was performed by re-suspending the liver parenchymal fraction in HBSS/10%FBS at 10⁷ cells/ml, followed by incubation with CD95-Biotin conjugate (1:100, eBioscience) for 30 min at 4°C. Excess unbound primary antibody was removed via washing and centrifugation at 400 × g for 10 min at 4°C; the resulting cell pellet was resuspended in 90 μl of de-gassed MACS buffer/10⁷ total cells, followed by magnetic labeling with Anti-Biotin micro-beads (Miltenyi Biotec Inc., Auburn, CA) per the manufacturer's specifications. Magnetic separation was performed using an appropriate MACS column in the magnetic field of a VarioMACS separator, with CD95-expressing primary hepatocytes collected as eluate (Fig. 1B). Hepatocytes were assessed for cell surface marker expression by staining with the following rat anti-mouse antibody conjugates (Table S1): CD95-APC, EpCAM/CD326-PE-Cy7, CD45-eFluor 780, and c-Kit-PE (1:100; eBioscience). Single cell suspensions were washed at 50 × g and 4°C for 3 min, and resuspended in HBSS/10% FBS with 10 μg/ml PI, followed by flow cytometry using a FACSAria cell sorter.

Marcelo K.L., Lin F., Rajapakshe K., Dean A., Gonzales N., Coarfa C., Means A.R., Goldie L.C, & York B. (2015). Deciphering hepatocellular responses to metabolic and oncogenic stress. Journal of biological methods, 2(3), e28.

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Cardiac Progenitor Cell Phenotyping

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Flow cytometry was performed on aggregates at day 3 of DE differentiation. Aggregates were dissociated with Accutase (Gibco #A1110501), washed with FACS buffer (0.5% BSA, 3 mM EDTA, PBS), and stained with CXCR4-APC (eBioscience #17-999-42), c-Kit-PE (eBioscience #12-1178-42), and EpCam-PE (BD #347198) at the company-recommended dilution on ice for 30 min. The cells were then washed with FACS buffer and analyzed using the MACSQuant^® Analyzer 10. FlowJo V10 software was used to analyze the data.

Sahabian A., Sgodda M., Naujok O., Dettmer R., Dahlmann J., Manstein F., Cantz T., Zweigerdt R., Martin U, & Olmer R. (2019). Chemically-Defined, Xeno-Free, Scalable Production of hPSC-Derived Definitive Endoderm Aggregates with Multi-Lineage Differentiation Potential. Cells, 8(12), 1571.

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