One million splenocytes from mixed BM chimeric mice were incubated in 96-well tissue culture plates coated with anti-NKp46 (29A1.4) as described (5 (link)), or with 10 ng/ml mouse IL-12 plus 10 ng/ml mouse IL-18 (R&D Systems), 10 ng/ml PMA plus 1 μg/ml ionomycin (Sigma-Aldrich), or co-cultured with 1 × 105 RMA or m157-transfected RMA cells as described (6 (link)). One million CellTrace Violet-labeled splenocytes were co-cultured with 1 × 105 RMA or m157-transfected RMA cells (fixed in 1% paraformaldehyde) in the absence or presence of 25 ng/ml mouse IL-33 (R&D) and/or 10 ng/ml mouse IL-12 with 50 U/ml human IL-2 (NCI) for 4 days at 37° C.
Mouse il 33
Manufactured by R&D Systems
Mouse IL-33 is a recombinant protein that represents the full-length mature form of the mouse Interleukin-33 (IL-33) cytokine.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse il 33
1
Activation and Expansion of NK Cells
2
Airway Inflammation Analysis in Mice
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WT or Slc15a4−/− mice were sensitized with 25 ng of mouse IL-33 (R&D Systems) in combination with 100 μg OVA administered intraperitoneally (i.p.) on day 0 (d0), boosted on d7 and challenged intratracheally with 100 μg OVA from d17 to d19 (3 consecutive days). Bronchioalveolar lavage or peripheral blood was collected for flow cytometric analysis or measurement of antigen-specific antibody production, respectively, at d20. OVA-specific antibody production was measured as described below.
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