Cf200 cu 25

Manufactured by Electron Microscopy Sciences

Sourced in United States

The CF200-Cu-25 is a copper specimen grid used in electron microscopy. It is a square-shaped grid made of copper and measures 200 mesh with a bar width of 25 micrometers. The grid is designed to support thin samples for examination under an electron microscope.

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Lab products found in correlation

Jem 2100 lab6 tem, by JEOL (2 mentions) Uranyl acetate replacement stain, by Electron Microscopy Sciences (1 mentions) Em grade paraformaldehyde, by Electron Microscopy Sciences (1 mentions)

2 protocols using cf200 cu 25

Negative Staining of Extracellular Vesicles

Cited 1 time

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The EVs were negatively stained using a protocol, as previously described [54 (link)]. Briefly, 4% paraformaldehyde (10 μL) was added to the EVs (10 μL), which incubated for 30 min. A carbon film grid (Electron Microscopy Sciences; Baltimore, MD, USA; CF200-Cu-25) was placed on the paraformaldehyde/EV droplet for 20 min and washed with PBS. Then, the grid was placed on 1% glutaraldehyde (50 μL) for 5 min and washed eight times with water. Finally, the grid was placed on uranyl acetate replacement stain (50 μL) for 10 min and left to dry for 10 min. The images were acquired on a JEOL JEM 2100 LaB6 TEM at 200 kV (40,000× magnification) using a digital camera (Gatan; Pleasanton, CA, USA).

Pottash A.E., Levy D., Jeyaram A., Kuo L., Kronstadt S.M., Chao W, & Jay S.M. (2022). Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy. Non-Coding RNA, 8(5), 71.

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Visualizing Extracellular Vesicle Morphology via TEM

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EV morphology was visualized via transmission electron microscopy (TEM) using a negative staining technique. A portion of each EV sample (10 μl) was fixed in a 1:1 solution using 4% EM-grade paraformaldehyde (Electron Microscopy Sciences, 157-4-100) for 30 min at room temperature. A 10 μl droplet of the EV-PFA mixture was then allowed to adsorb to a carbon-coated copper grid (Electron Microscopy Sciences, CF200-Cu-25) for 20 min. After a brief wash using a of a drop of 1X PBS, the EV-coated grid was then placed on a drop of 1% glutaraldehyde (in 1X PBS) for 5 min. The grid was washed 5-7 times (2 min each wash) on deionized water droplets with blotting on filter paper between washes. The grid was then positioned on a droplet of uranyl-acetate replacement stain (Electron Microscopy Sciences, 22405) and allowed to dry completely for 10 min. Once prepared, the grids were imaged at 200 kV on a JEOL JEM 2100 LaB6 TEM.

Kronstadt S.M., Van Heyningen L.H., Aranda A, & Jay S.M. (2023). Assessment of anti-inflammatory bioactivity of extracellular vesicles is susceptible to error via media component contamination. Cytotherapy, 25(4), 387-396.

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