Fugene and dual specific luciferase assay kit

Manufactured by Promega

FuGene is a transfection reagent that facilitates the delivery of nucleic acids into eukaryotic cells. The Dual-Specific Luciferase Assay Kit is a tool for measuring the activity of two different luciferase reporter genes simultaneously within the same cell sample.

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Lab products found in correlation

Polybrene, by Merck Group (2 mentions) Anti ha peroxidase, by Merck Group (1 mentions) Anti flag m2 peroxidase, by Merck Group (1 mentions) Phosphor irf3, by Cell Signaling Technology (1 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal antibodies against flag and β actin, by Merck Group (1 mentions) Ergic 53 p58, by Merck Group (1 mentions) Recombinant ifn β, by R&D Systems (1 mentions) Hsv 1 kos strain, by National Collection of Authenticated Cell Cultures (1 mentions)

Close spelling variants of this product

Dual Luciferase Assay (854 citations) Dual luciferase kit (463 citations) FuGENE (436 citations) Luciferase assay (182 citations) Promega luciferase kits (5 citations) Fugene kit (3 citations)

2 protocols using fugene and dual specific luciferase assay kit

Innate Immune Signaling Pathway Analysis

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poly(I:C), R848, PGN, and Lipofectamine 2000 (InvivoGen); MPLA and LPS (Sigma); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); puromycin and EZ-link Psoralen–PEG₃–Biotin (Thermo); streptavidin agarose (Solulink); ELISA kits for murine IFN-β (PBL), IL-6, and TNFα (BioLegend).
Mouse monoclonal antibodies against HA (Origene), FLAG, and β-actin, anti-HA-Peroxidase, and anti-FLAG-M2-Peroxidase (Sigma) and rabbit monoclonal antibodies against TLR3, phosphor-IRF3, p65 (Cell Signaling Technology), phosphor-TBK1, TBK1, TRIF (Abcam), and IRF3 (Santa Cruz Biotechnology) were purchased from the indicated manufacturers. Antisera against ZCCHC3, RIG-I, and MDA5 were generated by immunizing rabbits or mice with purified recombinant ZCCHC3 (133–404), RIG-I(231–925), and MDA5(239–1020). HEK293 and HT1080 cells were obtained from ATCC.

Zang R., Lian H., Zhong X., Yang Q, & Shu H.B. (2020). ZCCHC3 modulates TLR3-mediated signaling by promoting recruitment of TRIF to TLR3. Journal of Molecular Cell Biology, 12(4), 251-262.

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Innate Immune Sensing Mechanism Protocol

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3′3′-cGAMP, Lipofectamine 2000 (InvivoGen); poly(dA:dT), DNA90, ISD, HSV60, HSV120 (Sangon Biotech); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin and VPS34-IN1 (Sigma); puromycin (Thermo); recombinant IFN-β (R&D Systems); ELISA kits for murine IFN-α and IFN-β (PBL) and IL-6 (Biolegend).
Mouse monoclonal antibodies against FLAG and β-actin (Sigma) and HA (Covance); rabbit monoclonal antibodies against phospho-TBK1, TBK1 and SNX8 (Abcam); MITA, VPS34, IRF3, phospho-IRF3, phospho-IκBα and phosphor-MITA (Ser366) (Cell Signaling Technology), rabbit polyclonal antibody against ERGIC-53/p58 (Sigma); Alexa Fluor 555 Mouse anti GM130 (BD Biosciences) were purchased from the indicated companies. Rabbit and mouse anti-SNX8 sera were raised against a recombinant human SNX8 protein.
HEK293 cells were provided by Dr. Gary Johnson (National Jewish Center). THP-1 cells and HeLa cells were purchased from ATCC. HFFs were provided by Dr. Minhua Luo (Wuhan Institute of Virology). HSV-1 (KOS strain) (China Center for Type Culture Collection, Wuhan, China), vaccinia virus (Tian-Tan Strain) (China Center for Type Culture Collection, Wuhan, China) and ECTV (Han-Zhong Wang, Wuhan Institute of Virology, Wuhan, China) viruses have been obtained from the indicated resources.

Wei J., Lian H., Guo W., Chen Y.D., Zhang X.N., Zang R., Zhong L., Yang Q., Hu M.M., Luo W.W., Shu H.B, & Li S. (2018). SNX8 modulates innate immune response to DNA virus by mediating trafficking and activation of MITA. PLoS Pathogens, 14(10), e1007336.

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