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Tissue tek 2 oct compound

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Tissue-Tek II OCT compound is a cryoembedding medium used for the preparation of frozen tissue sections. It is designed to provide support and stabilization for tissue specimens during the freezing process prior to sectioning and analysis.

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Lab products found in correlation

Cryostat cm 1850, by Leica (1 mentions) Cryostat, by Leica (1 mentions)

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OCT compound (1065 citations) Tissue-Tek OCT compound (1023 citations) Tissue-Tek (717 citations) Tissue-Tek OCT (333 citations) Tissue-Tek compound (11 citations) Tissue-Tek® OCT Compound (OCT) (3 citations) Tissue-TEK II (2 citations)

2 protocols using tissue tek 2 oct compound

1

Soleus Muscle Cryosectioning Protocol

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Soleus muscles were dissected from mice, rinsed in PBS and gently dried, immersed in Tissue-Tek II OCT compound (Sakura Finetek USA, Inc., Torrance, CA, USA), frozen in isopentane cooled in liquid nitrogen, and preserved at −80 °C till sectioning. Then, 10 μm thick transverse serial sections were obtained by using Leica cryostat (CM 1850, Leica Microsystem, Wetzlar, Germany). Sections were airdried and stored at −80 °C till their utilization for histological analyses.
Toniolo L., Gazzin S., Rosso N., Giraudi P., Bonazza D., Concato M., Zanconati F., Tiribelli C, & Giacomello E. (2024). Gender Differences in the Impact of a High-Fat, High-Sugar Diet in Skeletal Muscles of Young Female and Male Mice. Nutrients, 16(10), 1467.
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2

Cryopreservation of Mouse Tibialis Anterior

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Tibialis anterior muscles were dissected from mice, directly frozen in isopentane cooled in liquid nitrogen, and cryoprotected with Tissue-Tek II OCT compound (Sakura Finetek USA, Inc., Torrance, CA, USA). Transverse serial sections, 8 μm thick, were cut with a Leica cryostat (CM 1850, Leica Microsystem, Wetzlar, Germany).
Giacomello E., Crea E., Torelli L., Bergamo A., Reggiani C., Sava G, & Toniolo L. (2020). Age Dependent Modification of the Metabolic Profile of the Tibialis Anterior Muscle Fibers in C57BL/6J Mice. International Journal of Molecular Sciences, 21(11), 3923.
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