Genomic DNA was extracted from whole blood using the Autopure LS (Qiagen, Hilden, Germany). All three single-nucleotide polymorphisms (SNP; PNPLA3 at rs738409, C>G/I148M; TM6SF2 at rs58542926, C>T/E167K; and MBOAT7 at rs641738, C>T) were genotyped by TaqMan PCR method (Applied Biosystems, Foster City, CA) according to manufacturer’s instructions. Post-PCR allelic discrimination was carried out measuring allele-specific fluorescence on an ABI Prism Sequence Detection System ABI 7900HT (Applied Biosystems). The success rate for genotyping was >95% for all three SNPs and the genotypes of all three SNPs were in Hardy–Weinberg equilibrium.
Taqman pcr method
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TaqMan PCR method is a real-time quantitative PCR (qPCR) technique used for the detection and quantification of specific DNA sequences. It utilizes a fluorescent probe that hybridizes to the target DNA, allowing for the monitoring of the amplification process in real-time. The core function of the TaqMan PCR method is to provide accurate and sensitive quantification of DNA targets.
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Lab products found in correlation
3 protocols using taqman pcr method
1
Genotyping of Liver Disease SNPs
2
Genotyping of PNPLA3 rs738409 Variant
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The PNPLA3 rs738409 (Ile148Met, C/G) was genotyped using the Taqman PCR method (Applied Biosystems, Foster City, CA USA), according to the manufacturer's instructions. ABI Prism Sequence Detection Systems ABI 7900HT (Applied Systems) was used for post-PCR allelic discrimination by allele-specific fluorescence. Genotypes were successfully determined for all individuals, and concordance rate of repeated genotyping of all individuals was 100%.
3
Genotyping of PNPLA3 rs738409 Variant
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PNPLA3 rs738409 was genotyped using Taqman PCR method (Applied Biosystems, Foster City, CA USA) according to manufacturer’s instructions. ABI Prism Sequence Detection Systems ABI 7900HT (Applied Systems) was used for post-PCR allelic discrimination by measuring allele-specific fluorescence. Genotypes were successfully determined for 96.5 % of the individuals, and the minor allele frequency was 21 %. The genotype distribution did not deviate from Hardy–Weinberg equilibrium (P = 0.26), and concordance rate of repeated genotyping of all individuals was 99.9 %.
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