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Eos 40d camera

Manufactured by Canon
Sourced in Japan

The EOS 40D is a digital single-lens reflex (DSLR) camera. It features a 10.1-megapixel CMOS sensor, DIGIC III image processor, and a 3.0-inch LCD screen. The camera supports multiple exposure modes, including manual, aperture priority, and shutter priority.

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6 protocols using eos 40d camera

1

Detailed Protocolo for Xanthopygina Taxonomy

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Photographs were taken using a Visionary Digital Passport System with a Canon EOS 40D camera and MP-E 65 lens. Images were automontaged using Helicon Focus 6.2.2. SEM photographs were taken using a Neoscope JEOL desktop SEM and processed using the Fluid Mask 3 software. All specimens were examined using an Olympus SZX10 stereomicroscope. Measurements were made using an ocular micrometer. Width: length ratio measurements were made on the widest and longest parts of the structure. The comparison between the length of the median lobe and the paramere excludes the bulbous basal part of the median lobe. Total body length is measured from the anterior margin of frons to the posterior margin of tergite VIII. Terminology and label data follow the procedure established by Ashe and Chatzimanolis 2003 and used in other Xanthopygina taxonomic works (e.g., Chatzimanolis 2004 , Chatzimanolis 2008 (link), Chatzimanolis 2012 (link), Chatzimanolis 2013 (link), Chatzimanolis 2014a (link), Chatzimanolis and Ashe 2009 ). The type locality of Scaponopselaphusmutator (Sharp) has been corrected as indicated in Asenjo et al. 2013 .
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2

Zoological Specimen Collection Analysis

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Specimens examined were obtained from or deposited in the collections of the following institutions:
ZISP

Zoological Institute of the Russian Academy of Sciences, Saint-Petersburg, Russia

ZMUM

Zoological Museum of the Lomonosov State University, Moscow, Russia

MNKB

Museum für Naturkunde, Leibniz-Institut für Evolutions und Biodiversitätsforschung, Berlin, Germany

Morphological terminology and abbreviations generally follow White et al. (1999) . Series of photos were taken directly by the Canon EOS 40D camera with Canon MP-E 65 mm lens and then composed with CombineZM software (Hadley 2007 ). Measurements are provided in millimetres.
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3

Wound Area Measurement using Digital Photography

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The wound area was photographed using a digital EOS40D camera (Canon, Tokyo, Japan) at one, three, six, and eight days post-wounding. The wound area was calculated using ImageJ software.
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4

Glass Slate Adherent Curing Protocol

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The specimens were manufactured as described in 2.2. Deviating from that description, transparent glass slates (100 × 100 × 2 mm) were used as adherents. The adherents were only cleansed with 2-propanol and not sandpapered. Curing did not take place under vacuum bagging conditions, but pressure plates were placed on each specimen to achieve good results. Photos were taken with a Canon EOS40D camera and an EF 50 mm/2.5 macro lens manufactured Canon (Tokyo, Japan) and cropped with the MATLAB Image Viewer. A MATLAB-based image recognition and analysis algorithm was used to analyze the percentage of air inclusions (or pores) in each sample.
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5

Wound Area Measurement using Digital Photography

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The wound area was photographed using a digital EOS40D camera (Canon, Tokyo, Japan) at one, three, six, and eight days post-wounding. The wound area was calculated using ImageJ software.
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6

Root Acid Phosphatase Staining and Activity

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Root APase staining was performed according to Bozzo's report [32] (link). The roots were excised from 7 days Pi-deprived and Pi-supplied seedlings and incubated with a 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) content agar solution containing 50 mM sodium acetate (pH 5.5) with 10 mM MgCl2, 0.6% agar and 0.08% BCIP at room temperature for 20 min. The blue color on the root surface, formed by hydrolysis of BCIP, was photographed using an EOS 40D camera (Canon Corporation, Tokyo, Japan). Protein (1 µg) was used for APase activity assay, and the protein was added to 620 µl of reaction buffer (50 mM NaAc pH 5.5 and 10 mM MgCl2), and 10 µl of p-nitrophenol phosphate (10 mg ml−1 pNPP; Sigma). After incubation at 37°C for 10 min, the reaction was stopped by 1.2 ml of 1M NaOH, and then absorbance was measured at 412 nm wavelength. Phosphatase activity was counted as ng of pNPP accumulated µg−1 soluble protein min−1. These experiments were repeated three times.
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