Aroclor 1254

Manufactured by AccuStandard

Sourced in United States

Aroclor 1254 is a polychlorinated biphenyl (PCB) compound manufactured and sold by AccuStandard for use in research and analytical applications. It is a viscous, yellow-colored liquid. Aroclor 1254 is a mixture of chlorinated biphenyl compounds with an average of 54% chlorine content by weight. The product is intended for use as a reference standard or analytical tool, and its specific applications should be determined by the user.

Automatically generated - may contain errors

Lab products found in correlation

Aroclor 1260, by AccuStandard (2 mentions) Aroclor 1248, by AccuStandard (1 mentions) Isoflo, by Abbott (1 mentions) Androstanol, by Merck Group (1 mentions) Pcb 126, by AccuStandard (1 mentions) Dimethyl sulfoxide (dmso), by Avantor (1 mentions) Dmem f12 medium, by Thermo Fisher Scientific (1 mentions) Nbt bcip, by Avantor (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Quercetin, by Merck Group (1 mentions) M mlv reverse transcriptase, by Promega (1 mentions) Rt pcr primers, by Sangon (1 mentions) D8418, by Merck Group (1 mentions)

7 protocols using aroclor 1254

Dose-dependent Sturgeon Embryo Exposure

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Shortnose sturgeon embryos (n = 25/treatment group, 3 d
post-fertilization) were water-borne exposed for 24 hr in 25 ml of 1-ppt
artificial sea water in 100-ml glass beakers to graded doses of TCDD (nominal
doses of 0.0005 parts per billion (ppb), 0.005 ppb, 0.05 ppb, 0.5 ppb, 5 ppb and
50 ppb (AccuStandard; 99.1% purity)); PCB77 (nominal doses of 0.1 ppb, 1.0 ppb,
10 ppb, 100 ppb, 1000 ppb, and 10,000 ppb (AccuStandard; 99.7% purity)); PCB81
(nominal doses of 0.1 ppb, 1.0 ppb, 10 ppb, 100 ppb, 1000 ppb, and 10,000 ppb
(AccusStandard 99.8% purity)); PCB126 (nominal doses of 0.1 ppb, 1.0 ppb, 10
ppb, 100 ppb, 1000 ppb, and 10,000 ppb (AccuStandard; 99.7% purity)); PCB169
(nominal doses of 0.1 ppb, 1.0 ppb, 10 ppb, 100 ppb, 1000 ppb, and 10,000 ppb
(AccuStandard; 99.0% purity)); and an Aroclor mixture of Aroclor 1248 (40%),
Aroclor 1254 (40%), and Aroclor 1260 (20%) (Accustandard) in acetone vehicle or
to acetone alone.
Shortnose sturgeon embryos were maintained in exposure water for 24 h at
12^oC after which they were rinsed and transferred to 750-ml Pyrex
dishes with 500 ml of clean 1 ppt seawater for rearing until hatch. Every 12 h,
dishes were cleaned of dead embryos, newly hatched larvae were removed, and an
80% percent water change was performed. Hatchlings were transferred and held
alive in beakers for 24 h, snap frozen in liquid nitrogen, and stored at
−80° C until RNA isolations.

Roy N.K., Torre M.D., Candelmo A., Chambers R.C., Habeck E, & Wirgin I. (2018). Characterization of AHR1 and its Functional Activity in Atlantic Sturgeon and Shortnose Sturgeon. Aquatic toxicology (Amsterdam, Netherlands), 205, 25-35.

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Prenatal PCB Exposure in Rats

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Timed pregnant wistar rats were housed individually under regulated temperature (22°C) and a 12-hr light/dark cycle. Pregnant rats were exposed orally to the commercial PCB mixture, Aroclor 1254 (Lot no. 124–191; AccuStandard, Inc., New Haven, CT), diluted in corn oil. Aroclor 1254 exhibit a congener profile similar to that found in human tissues and breast milk [21] . Dams were exposed daily (2:00–3:00 PM) to either Aroclor 1254 (6 mg/kg/day) or vehicle (corn oil), injected in one third of a wafer (Delacre, Groot-Bijgaarden, Belgium), from the sixth day of gestation (E6) until the last day of gestation. The doses were adjusted every other day to account for changes in body weight of the dams. Dams were anaesthetized with isoflurane (Isoflo, Abbott, UK) and trunk blood was collected after decapitation. Dams were divided into groups depending on age at sacrifice and exposition. Dams were sacrificed either at E17 or E19 to study cell proliferation, S phase and cell cycle exit, at E17 for cell death and neuronal differentiation rate and at E20 for neuronal migration, laminar organization and radial glia study. For each experiment, 3–6 embryos per group were used. Each embryo came from a different dam in order to avoid litter effects.

Naveau E., Pinson A., Gérard A., Nguyen L., Charlier C., Thomé J.P., Zoeller R.T., Bourguignon J.P, & Parent A.S. (2014). Alteration of Rat Fetal Cerebral Cortex Development after Prenatal Exposure to Polychlorinated Biphenyls. PLoS ONE, 9(3), e91903.

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TCPOBOP and Aroclor Effects on EGFR Signaling

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1,4-Bis(3,5-dichloro-2-pyridyloxy)benzene (TCPOBOP) and androstanol were obtained from Sigma Chemical Co., St. Louis, MO. Aroclor 1260, Aroclor 1254, and PCB congeners, PCB 3, PCB 6, PCB 8, PCB 9, PCB 126, PCB 138, PCB 149, PCB 151, PCB 153, PCB 170, PCB 174, PCB 180, PCB 187, were obtained from AccuStandard, Inc., New Haven, CT. Epidermal growth factor (EGF) and EGFR inhibitor (EI, Cyclopropanecarboxylic acid-(3-(6-(3-trifluoromethyl-phenylamino)-pyrimidin-4-ylamino)-phenyl)-amide) were purchased from Millipore (Norwood, OH). The following antibodies were used for Western blot analysis of cell lysates from HepG2 and AML-12 cells: EGFR (Santa Cruz, Santa Cruz, CA), P-EGFR Y1173 (abcam, Cambridge, MA), P-EGFR Y845 (abcam, Cambridge, MA), and P-EGFR Y1068 (CST, Danvers, MA), mouse liver EGFR, P-EGFR Y845, P-EGFR Y1068, mTOR, P-mTOR, AKT, P-AKT, STAT3, cRaf, ERK, P-ERK, β-actin, GAPDH (CST, Danvers, MA).

Hardesty J.E., Wahlang B., Falkner K.C., Clair H.B., Clark B.J., Ceresa B.P., Prough R.A, & Cave M.C. (2017). Polychlorinated Biphenyls Disrupt Hepatic Epidermal Growth Factor Receptor Signaling. Xenobiotica; the fate of foreign compounds in biological systems, 47(9), 807-820.

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TCDD and Aroclor1254 Exposure Protocol

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TCDD (lot number: ER052609) was obtained from Cerilliant Corporation (Cerilliant, Round Rock, TX, USA), and Aroclor1254 was obtained from AccuStandard (AccuStandard, New Haven, CT, USA). TCDD and Aroclor1254 were dissolved in a minimum amount of DMSO (Amresco, Solon, OH, USA) (0.1%) and diluted to the required concentration in corn oil (Fulinmen, China). The final concentration of DMSO in TCDD and Aroclor1254 solutions administered to mice was <0.01%. All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA) and were of analytical grade or of the highest grade available, unless otherwise mentioned.

Shan Q., Qu F, & Chen N. (2020). 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and Polychlorinated Biphenyl Coexposure Alters the Expression Profile of MicroRNAs in the Liver Associated with Atherosclerosis. BioMed Research International, 2020, 2652756.

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Aroclor 1254 and Quercetin Effects

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Aroclor 1254 was purchased from AccuStandard, Inc., New Haven, USA. Quercetin was purchased from Sigma-Aldrich Co., Louis, USA. CYP1A1 and CYP2B1 antibodies were purchased from Chemicon, USA. DMEM/F-12 medium and Trizol were purchased from Invitrogen, USA. Fetal bovine serum (FBS) was from HyClone, Logan, UT. NBT, BCIP were purchased from Amresco, USA. M-MLV reverse transcriptase was purchased from Promega, USA; RT-PCR primers were synthetized by Sangon Biotech Co. Ltd, Shanghai, China. TNF-α, IL-6, E₂, and P₄ ELISA kits were purchased from Biovalue, Shanghai, China.

Xu L., Sun L., Lu L., Zhong X., Ma Y, & Qin J. (2014). Effects of Quercetin on CYP450 and Cytokines in Aroclor 1254 Injured Endometrial Cells of the Pregnant Rats. BioMed Research International, 2014, 497508.

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Exposure to AhR Ligands in Corn Oil

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TCDD (99.8% purity; D-404S), Aroclor 1254 (99.8% purity; APP-9-163-10X), polychlorobiphenyl 126 (PCB126; 99.8% purity; C-126N), and benzo[

a

]pyrene (BaP; 99.8% purity; H169N) were obtained from AccuStandard, solubilized in dimethyl sulfoxide (DMSO; D8418; Sigma-Aldrich), and delivered in corn oil (405435000; Acros Organics/ThermoFisher). The control corn oil preparation delivered to rats included the same amount of DMSO used to initially solubilize the AHR ligands.

Iqbal K., Pierce S.H., Kozai K., Dhakal P., Scott R.L., Roby K.F., Vyhlidal C.A, & Soares M.J. (2021). Evaluation of Placentation and the Role of the Aryl Hydrocarbon Receptor Pathway in a Rat Model of Dioxin Exposure. Environmental Health Perspectives, 129(11), 117001.

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Aroclor 1254 Exposure in Astrocytes

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Aroclor 1254 (11097-69-1, AccuStandard, #C-254S-M) was diluted from a stock concentration of 1000 μg/mL in methanol. For exposure, Aroclor 1254 was diluted in serum-free astrocyte medium to the appropriate concentration as noted. Methanol was used as the vehicle (see figure legends for details).

McCann M.S., Fernandez H.R., Flowers S.A, & Maguire-Zeiss K.A. (2021). Polychlorinated biphenyls induce oxidative stress and metabolic responses in astrocytes. Neurotoxicology, 86, 59-68.

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