IHC analysis was conducted as previously described [27 (link)]. Deparaffinized tissue sections were treated with 3% hydrogen peroxide in methanol for 10 min to remove endogenous peroxidase. Antigen retrieval was performed in sodium citrate buffer (0.1 M) by using a microwave method. The slides were incubated with normal serum to block nonspecific binding and then incubated for 1 h with primary antibodies (1 : 100 to 1 : 200 dilutions) to TGF-β (MBS462142, MyBioSource), CCL-2 (PAB16617, Abnova, Taipei, Taiwan), CXCL1 (PAB8798, Abnova), and CXCL11 (bs-2552R, Bioss). The slides were incubated for 10 min with biotinylated secondary antibodies (PK-7800, Vector Laboratories, Burlingame, CA, USA) and horseradish peroxidase-conjugated streptavidin. The signals were detected by the application of the 3,3-diaminobenzidine tetrahydrochloride substrate chromogen solution and counterstaining with Mayer's hematoxylin. To evaluate the staining, after five circles of equal diameter had been drawn on separate areas, without overlapping, the positive cells were counted from four slides per group.
Pab16617
Manufactured by Abnova
Sourced in United Kingdom
PAB16617 is a laboratory product manufactured by Abnova. It is a device intended for use in scientific research and analysis, but its specific core function is not available without making interpretations or extrapolations beyond the factual information that can be provided concisely.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pab16617
1
Immunohistochemical Analysis of Cytokines
2
Immunohistochemical Analysis of Inflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An IHC analysis was conducted as previously described [10 (link)]. Deparaffinized tissue sections were treated with 3% hydrogen peroxide in methanol for 10 min to remove endogenous peroxidase. Antigen retrieval was carried out with sodium citrate buffer (0.1 M) using the microwave method. The slides were incubated with normal serum to block nonspecific binding and then incubated for 1 h with primary antibodies (diluted 1:100 to 1:200), such as antibodies against IFN-γ (sc-74104; Santa Cruz Biotechnology, Dallas, TX, USA), TNF-α (MBS175453; MyBioSource, San Diego, CA, USA), TGF-β (MBS462142; MyBioSource), IL-8 (ab110727; Abcam, Cambridge, UK), CCL-2 (PAB16617; Abnova, Taipei, Taiwan), CXCL-1 (PAB8798,; Abnova), CXCL-9 (bs-2551R; Bioss, Woburn, MA, USA), CXCL-10 (bs-1502R; Bioss), and CXCL-11 (bs-2552R; Bioss). The slides were incubated for 10 min with biotinylated secondary antibodies (PK-7800; Vector Laboratories, Burlingame, CA, USA) and horseradish peroxidase-conjugated streptavidin. Signals were detected using the 3,3-diaminobenzidine tetrahydrochloride substrate chromogen solution, and the cells were counterstained with Mayer's hematoxylin. In order to evaluate the expression levels, after 5 circles were drawn with equal diameters in separate areas, without overlap, the positive cells were counted from 4 slides per group.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!