Vimentin v9

Manufactured by Abcam

Sourced in United States

Vimentin V9 is a primary antibody that specifically recognizes the vimentin protein. Vimentin is a type III intermediate filament protein that is expressed in mesenchymal cells. The Vimentin V9 antibody can be used for the detection and localization of vimentin in various cell and tissue types.

Automatically generated - may contain errors

Lab products found in correlation

Laminin, by Santa Cruz Biotechnology (1 mentions) Integrin α6, by Abcam (1 mentions) Estrogen receptor, by Santa Cruz Biotechnology (1 mentions) Claudin 3, by Abcam (1 mentions) E cadherin, by Abcam (1 mentions) Flag tag m2, by Merck Group (1 mentions) α tubulin dm1a, by Merck Group (1 mentions) Gapdh ab9484, by Abcam (1 mentions) Ab1791, by Abcam (1 mentions) Erα hc 20, by Santa Cruz Biotechnology (1 mentions) Perk1 2 e 4, by Santa Cruz Biotechnology (1 mentions) E cadherin h 108, by Santa Cruz Biotechnology (1 mentions) Erk2 c 14, by Santa Cruz Biotechnology (1 mentions) 4 hydroxytamoxifen, by Merck Group (1 mentions) Tunicamycin, by Merck Group (1 mentions) 17β estradiol e2, by Merck Group (1 mentions)

2 protocols using vimentin v9

Protein Extraction and Western Blot Analysis

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Cells were washed in ice-cold phosphate-buffered saline and lysed in T-PER (tissue protein extraction reagent) supplemented with Halt protease and phosphatase inhibitor cocktail (all from Pierce/Life Technologies, Grand Island, NY, USA). Protein content was measured by Bradford Assay; 30–50 μg protein was resolved on 12% SDS–PAGE and transferred to nitrocellulose membranes. Membranes were blocked with 5% milk in TBST (0.1% Tween-20 in TBS) and incubated overnight with the following antibodies: PAR1-ATAP2, EGFR, estrogen receptor, Laminin (Santa Cruz, Dallas, TX, USA), Vimentin V9, E-cadherin, Integrin α6, Keratin 8/18, Claudin 3 (Abcam, Cambridge, MA, USA), Zona Occluden-3 (Chemicon, Billerica, MA, USA), SLUG, Zeb1 (Cell Signaling Technology, Danvers, MA, USA).

Yang E., Cisowski J., Nguyen N., O’Callaghan K., Xu J., Agarwal A., Kuliopulos A, & Covic L. (2015). Dysregulated protease activated receptor 1 (PAR1) promotes metastatic phenotype in breast cancer through HMGA2. Oncogene, 35(12), 1529-1540.

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Estrogen Receptor Signaling Assay

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17β-estradiol (E2), 4-hydroxytamoxifen (OHT), and tunicamycin were purchased from Sigma-Aldrich. E2 and OHT were dissolved in ethanol, tunicamycin in dimethyl sulfoxide (DMSO). Primary antibodies were the following: against the FLAG tag (M2, Sigma Aldrich), α-tubulin (DM1A, Sigma Aldrich), histone H3 (ab1791, Abcam), pERK1/2 (E-4, Santa Cruz Biotech), ERK2 (C-14, Santa Cruz Biotech), GAPDH (ab9484, Abcam), ERα (HC-20, Santa Cruz Biotech), GPER (LS-A4272, LifeSpan Biosciences), vimentin (V9, Abcam), and E-cadherin (H-108, Santa Cruz Biotech).

Pupo M., Bodmer A., Berto M., Maggiolini M., Dietrich P.Y, & Picard D. (2017). A genetic polymorphism repurposes the G-protein coupled and membrane-associated estrogen receptor GPER to a transcription factor-like molecule promoting paracrine signaling between stroma and breast carcinoma cells. Oncotarget, 8(29), 46728-46744.

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