Losartan potassium tablets

Male Wistar rats weighing 180–200 g were acquired from Beijing HFK Bioscience Co., Ltd., and fed at the experimental animal center of the Chinese PLA General Hospital at 24±2 °C and 50%±20% humidity. The rats were divided into the following three groups, with 24 rats within each group: (I) sham group, (II) anti-thy1.1 group (Thy-1 group) and (III) anti-thy1.1 nephritis model + losartan group (losartan group) in which losartan was given by gavage at a dose of 50 mg/kg body weight per day. On day 0, the rats in the sham group received 0.2 mL of 1× phosphate-buffered saline (pH 7.4) by tail intravenous injection. The rats in the Thy-1 group were given an intravenous injection of monoclonal anti-thy1.1 antibody (2.5 mg/kg) produced by OX-7 hybridoma cells diluted in 1× phosphate-buffered saline (pH 7.4) and were administered vehicle from days 0 to 14. The losartan group was injected with the same dose of anti-thy1.1 antibody as the Thy-1 group as above on day 0 and administered losartan potassium tablets (Merck Sharp & Dohme Corp.) dissolved in deionized water on days 0–14. Blood and urine samples were collected on days 3, 7, and 14 after modeling. In each group, eight rats were sacrificed on days 3, 7, and 14.

Investigational medicinal products were procured from Micro Labs Ltd., Bangalore, India, which included the test product, losartan potassium tablets 100 mg of Micro Labs Ltd., India and the reference product, Cozaar^® 100 mg of losartan potassium tablets of Merck and Co., INC., USA.

The Qi-Bang-Yi-Shen Formula (QBF) is composed of HQ, DH, SQ, NBZ, BS and black beans at a rate of 10:5:1:5:5:5 (w/w/w/w). These herbs were provided by the Jiangyin Tianjiang Pharmaceutical Co., Ltd. The Losartan potassium tablets was obtained from the Merck Sharp & Dohme Limited (Approval Number: National medicine standard word J20180054; Hangzhou).