The largest database of trusted experimental protocols

2 protocols using anti sox2 goat polyclonal

1

Immunofluorescence Analysis of Embryo Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Embryo isolation and staging were done as described previously4 (link). The immunofluorescence analysis and AP staining were performed essentially as described previously4 (link). The primary antibodies used were as follows: anti-AP2γ rabbit polyclonal, 1:500 (cat#sc-8977; Santa Cruz Biotechnology); anti-ETMT1/GLP mouse monoclonal, 5µg/mL (cat#PP-B0422-00; R&D Systems); anti-H3K9me2 mouse monoclonal, 1:500 (cat#ab1220; Abcam); anti-SOX2 goat polyclonal, 1:200 (cat#sc-17320, Santa Cruz Biotechnology). The following secondary antibodies from Molecular Probes (Eugene, OR) were used at a dilution of 1:500: Alexa Fluor 488 goat anti-rabbit IgG; Alexa Fluor 568 goat anti-mouse IgG. The stained embryos were mounted with VECTASHIELD Antifade Mounting Medium (cat#H-1000; VECTOR LABORATORIES). The immunofluorescence images and the AP staining images were taken by a confocal microscope (Zeiss LSM880) and a stereomicroscope (Leica M80), respectively. The image analyses were done by using ImageJ/Fiji software.
+ Open protocol
+ Expand
2

Immunofluorescence Analysis of Embryo Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Embryo isolation and staging were done as described previously4 (link). The immunofluorescence analysis and AP staining were performed essentially as described previously4 (link). The primary antibodies used were as follows: anti-AP2γ rabbit polyclonal, 1:500 (cat#sc-8977; Santa Cruz Biotechnology); anti-ETMT1/GLP mouse monoclonal, 5µg/mL (cat#PP-B0422-00; R&D Systems); anti-H3K9me2 mouse monoclonal, 1:500 (cat#ab1220; Abcam); anti-SOX2 goat polyclonal, 1:200 (cat#sc-17320, Santa Cruz Biotechnology). The following secondary antibodies from Molecular Probes (Eugene, OR) were used at a dilution of 1:500: Alexa Fluor 488 goat anti-rabbit IgG; Alexa Fluor 568 goat anti-mouse IgG. The stained embryos were mounted with VECTASHIELD Antifade Mounting Medium (cat#H-1000; VECTOR LABORATORIES). The immunofluorescence images and the AP staining images were taken by a confocal microscope (Zeiss LSM880) and a stereomicroscope (Leica M80), respectively. The image analyses were done by using ImageJ/Fiji software.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!