6470 triple quadruple mass spectrometer

The determination of HCPT was performed on an Agilent 1290 series liquid chromatography system and an Agilent 6470 triple-quadruple mass spectrometer (Palo Alto, CA). The HPLC/MS conditions and sample preparation were basically according to a validated HPLC method described before (Zhang et al. 2019 ). The chromatographic analysis of HCPT was performed on a Waters X-Bridge C18 column (3.0 × 100 mm, i.d.; 3.5 μm, Waters Corporation, Milford, MA ) at room temperature (25 °C). The mobile phase was water (containing 0.1% formic acid) and acetonitrile (30:70, v: v) with isocratic elution at a flow rate of 0.2 mL/min, and the analysis time was 4 min.
The mass scan mode was positive MRM mode. The precursor ion and product ion are m/z 365.20→321.10 for HCPT and m/z 349.25→305.15 for IS. The collision energy for HCPT and IS were 30 and 20 eV, respectively. The MS/MS conditions were optimized as follows: fragmentor, 110 V; capillary voltage, 3.5 kV; Nozzle voltage, 500 V; nebulizer gas pressure (N₂), 40 psig; drying gas flow (N₂), 10 L/min; gas temperature, 350 °C; sheath gas temperature, 400 °C; sheath gas flow, 11 L/min.

All experiments were carried out with an Agilent 1290 HPLC coupled with 6470 triple quadruple mass spectrometer (Palo Alto, CA, USA). Data were acquired and processed by Agilent MassHunter Workstation 10.1 (Palo Alto, CA, USA). HB-Z303-1AC High-voltage DC Power supply (Tianjin, China) and KQ-500DA CNC ultrasonic cleaner (Kunshan, China) were used. Grade 1 chromatographic paper was from Whatman (Stevenage, UK).

All experiments were carried out with an Agilent 1290 HPLC coupled with a 6470 triple quadruple mass spectrometer (Palo Alto, CA, USA). Data were acquired and processed by Agilent MassHunter Workstation 10.1 (Palo Alto, CA, USA). HB-Z303-1AC High-voltage DC Power supply (Tianjin, China) and KQ-500DA CNC ultrasonic cleaner (Kunshan, China) were used. Grade 1 chromatographic paper was from Whatman (Stevenage, UK).