8201 pc spectrometer

(9Z)-octadec-9-enoic (97%) and Undec-10-enoic (98%) acids were purchased from fluka chemicals (Bucks, Switzerland). The 4-Methoxybenzylamine was purchased from TCI chemicals (India) Pvt. Ltd. All the solvents used were of analytical grade. The glass plates with layer of silica gel (0.5 mm, Merck, Mumbai, India) were used for thin layer chromatography (TLC). Highly polymerized Calf thymus DNA (ctDNA) was obtained from Sigma Aldrich. Hoechst33342 was obtained from Life Technologies, USA. Ethidium bromide (EtBr) was purchased from SRL. Tris–HCl buffer medium (10 mM) at pH 6.8 was used as medium for conducting experiments. The EtBr (0.1 mM) and HO stock solutions (0.15 mM) after being prepared by dissolving their crystals in a Tris–HCl buffer solution have been stored in a cool and dark place. Column chromatography was conducted using silica gel (Merck, Mumbai, India) having mesh size of 60–120. The Shimadzu 8201 PC spectrometer was used to record IR spectra and absorption as given in cm-1. 13C NMR and 1H NMR were recorded in Bruker DRX-400 instrument in CDCl3. TMS was used as an internal standard, and chemical shifts (δ) were measured relative to TMS and quoted in ppm. Coupling constants (J) are expressed in Hertz (Hz). The JEOL-SX 102/DA-600 Mass spectrometer was used to record mass spectra. Melting points are taken in an open capillary and are uncorrected.

All chemicals were purchased from Merck and Sigma–Aldrich, and were used without further purification. Pre-coated silica gel plates impregnated with a fluorescent indicator were used for analytical TLC. An ethyl acetate/hexane solvent mixture was used as the eluent for both TLC and column chromatography. The FT–IR spectra were recorded using KBr pellets on a Shimadzu 8201pc spectrometer operating between 4000 and 400 cm⁻¹. The ¹H and ¹³C NMR spectra were obtained on Bruker DRX-300 MHz and Bruker DRX-75 MHz Avance spectrometers, respectively, and the resulting chemical shifts were expressed in ppm using tetramethylsilane as the internal standard. JEOL JMS D-300 spectrometer was used to analysis the mass spectra (EI) all compounds. Elemental analysis (C, H, N, and S) was conducted using a Varian EL III elemental analyzer.