Water-compatible commercial molecularly imprinted polymers (MIPs) material, named SupelMIPTM SPE-Fluoroquinolones, was purchased from Sigma–Aldrich (St.Louis, MO, USA). Fe3O4 magnetite nanocomposites (MNPs) were prepared according to previous studies46 (link),66 (link) (detailed in Supplementary). Fluoroquinolones (norfloxacin, enoxacin, and fleroxacin, purity 98%) were purchased from J&K Scientific Ltd. (Shanghai, China). The individual stock solutions of norfloxacin, enoxacin, and fleroxacin were prepared in methanol at a concentration of 0.1 mg mL−1 and stored at 4 °C before use. Both Methanol and acetonitrile were HPLC grade and purchased from Merck KGaA (Darmstadt, Germany). Ammonium hydroxide solution (suitability for use in UPLC/LC-MS, NH3, w/w 20%) was bought from CNW Technologies GmbH (Düsseldorf, Germany). Ethylene glycol, ethylene diamine, ferric trichloride hexahydrate (FeCl3·6H2O), and sodium acetate (NaAc) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Ultrapure water obtained from a Millipore water purification system (Milli-Q, Millipore; Bedford, MA, USA).
Fleroxacin
Manufactured by J&K Scientific
Sourced in China
Fleroxacin is a broad-spectrum fluoroquinolone antibiotic used for the treatment of various bacterial infections. It functions by inhibiting bacterial DNA gyrase and topoisomerase IV, which are essential enzymes for bacterial DNA replication and cell division.
Automatically generated - may contain errors
Lab products found in correlation
Norfloxacin, by J&K Scientific (2 mentions)
Milli q, by Merck Group (1 mentions)
Sodium acetate naac, by Sinopharm (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Ethylene glycol, by Sinopharm (1 mentions)
Ethylenediamine, by Sinopharm (1 mentions)
Ciprofloxacin, by J&K Scientific (1 mentions)
Ofloxacin, by J&K Scientific (1 mentions)
Hplc grade ethanol, by Merck Group (1 mentions)
Milli q ultrapure water, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Enrofloxacin, by J&K Scientific (1 mentions)
2 protocols using fleroxacin
1
Magnetic Molecularly Imprinted Polymers for Fluoroquinolone Analysis
2
Analytical Standards for Quinolone Antibiotics
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Analytical standards for fleroxacin (FLE), ofloxacin (OFL), norfloxacin (NOR), ciprofloxacin (CIP) and enrofloxacin (ENR) were purchased from J&K Chemical, China and used without further purification. HPLC-grade ethanol, methanol, ethyl acetate, acetonitrile and acetone were sourced from Merck (www.merck.com.cn). Salts (magnesium sulfate (MgSO 4 ), sodium sulfate (Na 2 SO4), sodium acetate (CH 3 COONa), ammonium sulfate ((NH 4 ) 2 SO 4 ) and ammonium acetate (CH 3 COONH 4 )) with purities ≥99% were obtained from Aladdin Industrial Co. Ltd.
Stock standard solutions (1000 g mL -1 ) for each FQ were prepared by dissolving each compound in methanol and stored at 4 • C. Stock solutions were diluted with methanol to prepare a secondary mixed stock solution of 10 g mL -1 . Mixtures of standard working solutions for extraction at different concentrations were prepared daily by dilution with Milli-Q ultrapure water (Millipore, Bedford, USA).
Stock standard solutions (1000 g mL -1 ) for each FQ were prepared by dissolving each compound in methanol and stored at 4 • C. Stock solutions were diluted with methanol to prepare a secondary mixed stock solution of 10 g mL -1 . Mixtures of standard working solutions for extraction at different concentrations were prepared daily by dilution with Milli-Q ultrapure water (Millipore, Bedford, USA).
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