Sfn cys

SFN-Cys and SFN-NAC were purchased from Santa Cruz Biotechnology (USA). PTX (Taxol, as a brand name) was obtained from Selleckchem (USA). Anti-Caspase-3, anti-β-actin, anti-α-tubulin, anti-Tau and protein A/G PLUS agarose were purchased from Santa Cruz Biotechnology (USA). Anti-βIII-tubulin and anti-Caspase-7 were purchased from Abcam (USA). Anti-Hsp70, anti-XIAP, anti-ERK1/2 and anti-pERK1/2 (Thr202/Tyr204) were obtained from Cell Signaling Technology (USA). Anti-Stathmin1 was obtained from Sangon Biotech Co.Ltd. (Shanghai, China). Anti-cleaved-PARP and anti-β-tubulin were purchased from Wanleibio (Shenyang, China). Annexin V-FITC/PI apoptosis assay kit was purchased from NeoBioscience (Shenzhen, China). Recombinant human Caspase-3 was purchased from Sino Biological Inc. (Beijing, China).

SFN-Cys was the product of Santa Cruz Biotechnology (Santa Cruz, CA, USA). BCA protein assay kit was purchased from Invitrogen (Carlsbad, CA, USA). Annexin V-FITC apoptosis assay kit was purchased from GenStar (Beijing, China). Antibody against caspase-3 (1 : 200) and antibody against β-actin (1 : 5000) were purchased from ProteinTech Group, Inc. (Chicago, IL, USA). Anti-ERK1/2 (1 : 1000), anti-phospho-ERK1/2 (1 : 1000) and phosphorylated ERK1/2 inhibitor, PD98059 were purchased from Cell Signaling Technology, Inc. (Shanghai, China). Anti-maspin (1 : 1000), anti-Bax (1 : 1000), anti-α-tubulin (1 : 5000) and anti-Bcl-2 (1 : 500) were purchased from Sangon, Ltd (Shanghai, China). Recombinant caspase-3 was purchased from Sino Biological Inc. (Beijing, China). MTS assay kit was purchased from Promega Company (Madison, WI, USA).

SFN-Cys and SFN-NAC were purchased from Santa Cruz Biotechnology (CA, USA). Lipofectamine^TM RNAiMAX was purchased from Invitrogen-Life Technologies (CA, USA). Anti-ERK1/2 (1:1000), anti-phospho-ERK1/2 (1:1000), and phosphorylated ERK1/2 inhibitor PD98059 were purchased from Cell Signaling Technology, Inc. (Shanghai, China). Anti-Claudin-1 (1:2000) was purchased from Abcam (MA, USA), anti-Claudin-5 (1:1000) was purchased from Santa Cruz (CA, USA), and anti-Claudin-7 (1:100) was purchased from Sangon Biotech, Ltd (Shanghai, China). Anti-LC3 was purchased from Cell Signaling Technology (CO, USA). Anti-Tau (Tau46) and anti-α-tubulin (B-7) were purchased from Santa Cruz (TX, USA). Autophagy inhibitor 3-Methyladenine (3-MA) was bought from Sigma (Hongkong, China) and Bafilomycin A1 (Baf-1) was from Selleck (Shanghai, China).