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Inform v2.4.8

Manufactured by PerkinElmer

InForm (v2.4.8) is a software solution designed for data analysis and visualization in life science research. It provides tools for managing and analyzing large datasets, with features for image processing, quantitative analysis, and data integration.

Automatically generated - may contain errors

2 protocols using inform v2.4.8

1

Multiplex Immunostaining for EBV Markers in FFPE Tissue

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Spleen and liver sections were fixed in 4% formalin and paraffin-embedded (FFPE). Immunohistochemical single staining of EBNA2 (clone PE2, Abcam) and LANA (clone LN53, Clinisciences) was carried out on a Leica BOND-III automated immunohistochemistry system using diaminobenzidin (DAB) as chromogen (Zytomed Systems, Berlin, Germany). In situ-hybridization for the detection of EBER was performed as described previously (Meyer et al., 2011 (link)) with DAP as substrate. Multiplex immunofluorescence (IF) staining was performed on FFPE tissue using Opal dyes and Spectral DAPI (FP1490) from PerkinElmer. Opal dyes 520, 540, 620 and 690 were used to detect EBNA2 (clone PE2, Abcam), CD20 (clone SP32, Cell Marque), LANA (clone LN53, CliniSciences) and IRF4/Mum1 (clone: MUM1p, CliniSciences), respectively. Single stains were used to compensate overlapping spectras. Staining was quantified on a Vectra3 automated quantitative pathology imaging system using Vectra, Phenochart (v1.0) and InForm (v2.4.8) software (all from PerkinElmer), as previously described (Murer et al., 2018 (link)).
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2

Multiplex Immunostaining for EBV Markers in FFPE Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleen and liver sections were fixed in 4% formalin and paraffin-embedded (FFPE). Immunohistochemical single staining of EBNA2 (clone PE2, Abcam) and LANA (clone LN53, Clinisciences) was carried out on a Leica BOND-III automated immunohistochemistry system using diaminobenzidin (DAB) as chromogen (Zytomed Systems, Berlin, Germany). In situ-hybridization for the detection of EBER was performed as described previously (Meyer et al., 2011 (link)) with DAP as substrate. Multiplex immunofluorescence (IF) staining was performed on FFPE tissue using Opal dyes and Spectral DAPI (FP1490) from PerkinElmer. Opal dyes 520, 540, 620 and 690 were used to detect EBNA2 (clone PE2, Abcam), CD20 (clone SP32, Cell Marque), LANA (clone LN53, CliniSciences) and IRF4/Mum1 (clone: MUM1p, CliniSciences), respectively. Single stains were used to compensate overlapping spectras. Staining was quantified on a Vectra3 automated quantitative pathology imaging system using Vectra, Phenochart (v1.0) and InForm (v2.4.8) software (all from PerkinElmer), as previously described (Murer et al., 2018 (link)).
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