Blinded to the IgG4 quantification, two authors (C.K. and S.G.A.) analyzed one haematoxylin and eosin (H&E) slide from each case sectioned from the same paraffin-embedded tissue cassette as were the immunostained slides. The parameters assessed were: a dense lymphoplasmacytic infiltrate, storiform fibrosis, obliterative phlebitis, epithelioid histiocytes, granulomas, giant cells, and neutrophils. Immunohistochemistry staining was performed as described previously (24 (link)). Specific to this study, slides were incubated for 1.5 hours at room temperature with an antibody cocktail containing rabbit anti-IgG (Cell Marque, Cat# 269A-15) diluted 1:5400 and mouse anti-IgG4 (Cell Marque, Cat# 367M-15) diluted 1:900 and detected with Mach 2 Double Stain 2, (Biocare Medical) for 10 minutes at room temperature.
Rabbit anti igg
Manufactured by Cell Marque
Rabbit anti-IgG is a laboratory reagent used for the detection and identification of immunoglobulin G (IgG) in various immunological and biochemical applications. It is a polyclonal antibody raised in rabbits against human IgG. This reagent can be used in techniques such as Western blotting, ELISA, and immunohistochemistry to study the presence and distribution of IgG in biological samples.
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Lab products found in correlation
2 protocols using rabbit anti igg
1
Immunostaining Protocol for IgG4 Quantification
2
Immunohistochemical Quantification of IgG4 Levels
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Blinded to the IgG4 quantification, two authors (C.K. and S.G.A.) analyzed one hematoxylin and eosin slide from each case sectioned from the same paraffin‐embedded tissue cassette as were the immunostained slides. The parameters assessed were: a dense lymphoplasmacytic infiltrate, storiform fibrosis, obliterative phlebitis, epithelioid histiocytes, granulomas, giant cells, and neutrophils. Immunohistochemistry staining was performed as described previously 24 . Specific to this study, slides were incubated for 1.5 hours at room temperature with an antibody cocktail containing rabbit anti‐IgG (Cat# 269A‐15; Cell Marque, Rocklin, CA) diluted 1:5400 and mouse anti‐IgG4 (Cat# 367M‐15; Cell Marque) diluted 1:900 and detected with Mach 2 Double Stain 2 (Biocare Medical, Concord, CA) for 10 minutes at room temperature.
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