Images were collected using a custom-built spinning disk confocal microscope (Nobska Imaging), which was configured for automation with Metamorph software (Molecular Devices). This confocal consists of a Hamamatsu ORCA EM-CCD camera mounted on an upright Zeiss Axio Imager.A2 with a Borealis-modified Yokogawa CSU-10 spinning disk scanning unit and a Zeiss Plan-Apochromat 100x/1.4 oil DIC objective. Animals were anesthetized for imaging by picking them into a drop of M9 on a 5% agarose pad containing 7 mM sodium azide and secured with a coverslip.
Orca em ccd camera
Manufactured by Zeiss
The ORCA EM-CCD camera from Zeiss is a high-sensitivity, low-noise detector designed for demanding imaging applications. It utilizes an electron-multiplying CCD sensor to enhance signal detection, making it suitable for a variety of microscopy techniques.
Automatically generated - may contain errors
Lab products found in correlation
Axio imager a2, by Zeiss (3 mentions)
Plan apochromat 100x 1.4 oil dic objective, by Zeiss (2 mentions)
Csu 10, by Zeiss (2 mentions)
Metamorph software, by Molecular Devices (2 mentions)
Plan apochromat 100x 1.4 na oil dic objective, by Zeiss (1 mentions)
Axiocam mrm camera, by Zeiss (1 mentions)
Csu10, by Yokogawa (1 mentions)
3 protocols using orca em ccd camera
1
Automated Spinning Disk Confocal Imaging
2
Spinning Disk Confocal Imaging of C. elegans
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All micrographs included in this manuscript were collected on a Hamamatsu Orca EM-CCD camera mounted on an upright Zeiss AxioImager A2 with a Borealis-modified CSU10 Yokagawa spinning disk scan head using 405nm, 488 nm, and 561 nm Vortran lasers in a VersaLase merge and a Plan-Apochromat 100x/1.4 (NA) Oil DIC objective. MetaMorph software (Molecular Devices) was used for microscopy automation. Several experiments and all RNAi screening were scored using epifluorescence visualized on a Zeiss Axiocam MRM camera, also mounted on an upright Zeiss AxioImager A2 and a Plan-Apochromat 100x/1.4 (NA) Oil DIC objective. Animals were mounted into a drop of M9 on a 5% Noble agar pad containing approximately 10 mM sodium azide anesthetic and topped with a coverslip.
3
Spinning Disk Confocal Imaging of C. elegans
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Images were acquired using a spinning disk confocal microscope supported by Nobska Imaging. This confocal system consists of a Hamamatsu ORCA EM-CCD camera mounted on an upright Zeiss Axio Imager.A2, equipped with a Borealis-modified Yokogawa CSU-10 spinning disk scanning unit with 6 solid state (405, 440, 488, 514, 561, and 640 nm) lasers and a Zeiss Plan-Apochromat 100x/1.4 oil DIC objective.
For static imaging, animals were anesthetized by placing them into a drop of M9 on a 5% agarose pad containing 7 mM sodium azide and securing them with a coverslip. Time-lapse imaging was performed using a modified version of a previously published protocol (Kelley et al., 2017 (link)), as described in Adikes et al. (2020) (link).
For static imaging, animals were anesthetized by placing them into a drop of M9 on a 5% agarose pad containing 7 mM sodium azide and securing them with a coverslip. Time-lapse imaging was performed using a modified version of a previously published protocol (Kelley et al., 2017 (link)), as described in Adikes et al. (2020) (link).
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