Simvastatin, cisplatin and mevalonic acid lactone were purchased from Sigma-Aldrich (Merck KGaA). For in vitro administration, Simvastatin was dissolved in dimethyl sulfoxide (DMSO) at a concentration of 50 mM and stored at −20°C as a stock solution. mevalonic acid lactone was dissolved in PBS at a concentration of 200 mM as stock solution. C666-1 was purchased from American Type Culture Collection (Thermo Fisher Scientific, Inc.) and cultured in RPMI-1640 (Corning Inc.) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin (Sigma-Aldrich; Merck KGaA). Cells were cultured at 37°C in a humidified atmosphere of 5% CO2. In the signal pathway experiments, cells were pretreated with JNK inhibitor, SP6000125 (10 µM; BioSource; Thermo Fisher Scientific, Inc.), 4 h before Simvastatin treatment.
Mevalonic acid lactone
Manufactured by Merck Group
Sourced in United States
Mevalonic acid lactone is a chemical compound used in laboratory settings. It is a cyclic ester derived from mevalonic acid, a key intermediate in the biosynthesis of various isoprenoid compounds. Mevalonic acid lactone serves as a versatile research tool for studies related to isoprenoid metabolism and signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Simvastatin, by Thermo Fisher Scientific (1 mentions)
Simvastatin, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Rpmi 1640, by Corning (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
C666 1, by American Type Culture Collection (1 mentions)
Ubiquinone, by Merck Group (1 mentions)
Rapamycin, by Fujifilm (1 mentions)
Isopentenyladenine, by Merck Group (1 mentions)
Squalene, by Merck Group (1 mentions)
U0126, by Promega (1 mentions)
Syringe filters, by Iwaki (1 mentions)
2 protocols using mevalonic acid lactone
1
Simvastatin and Cisplatin in C666-1 Cells
2
Lipid Biosynthesis Inhibitor Screening
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Fluvastatin, simvastatin, and MG132 were purchased from Calbiochem (San Diego, CA, USA). Mevalonic acid lactone (MVA), FPP, GGPP, squalene, ubiquinone, isopentenyladenine, and dolichol were purchased from Sigma (St. Louis, MO, USA). U0126 was purchased from Promega (Madison, WI, USA). Rapamycin, z-VAD-fmk, z-LEHDfmk, and z-DEVE-fmk were purchased form Wako (Osaka, Japan). These reagents were dissolved in dimethyl sulfoxide. And then, these dissolved reagents were resuspended in phosphate-buffered saline (PBS; 0.05 M, pH 7.4) and filtrated through syringe filters (0.45 μm; IWAKI GLASS, Tokyo, Japan) before use.
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