HPLC-grade petroleum ether, methanol, dimethyl sulfoxide (DMSO), and ethyl acetate were purchased from Merck (Darmstadt, Germany). Antibiotics (streptomycin and penicillin), 0.25% trypsin with EDTA in HBSS, high-glucose Dulbecco's modified Eagle medium (DMEM), and fetal bovine serum (FBS) were purchased from Biowest (Nuaillé, France). Plasmocin prophylactic for mycoplasma growth inhibition was purchased from InvivoGen (San Diego, CA, USA). Antibodies for TYR (SC-7834), TYRP-1 (SC-10448), TYRP-2 (SC-10451), and MITF (SC-10999) and anti-goat antibodies (SC-2020) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). p-ERK (9101S), ERK (4695S), p-CREB (9198S), CREB (4820S), p-AKT (4060S), AKT (9272S), p-mTOR (5536S), mTOR (2972S), PI3K (4292S), and anti-mouse (7076S) and anti-rabbit (7074S) antibodies were purchased from Cell Signaling (Danvers, MA, USA). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH, ab8245), Alexa Fluor® 568 (ab175471), p-PI3K (ab182651), and Alexa Fluor® 488 (ab150113) were purchased from Abcam (Cambridge, MA, USA). All standards for chromatography were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Hplc grade petroleum ether
Manufactured by Merck Group
Sourced in Germany
HPLC-grade petroleum ether is a highly purified solvent used in analytical and preparative high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile, and flammable hydrocarbon mixture. The product meets the stringent purity requirements for HPLC-grade solvents, ensuring consistent and reliable performance in HPLC systems.
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Lab products found in correlation
Fetal bovine serum (fbs), by Biowest (1 mentions)
Sc 2020, by Santa Cruz Biotechnology (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh ab8245, by Abcam (1 mentions)
High glucose dulbecco s modified eagle medium dmem, by Biowest (1 mentions)
Alexa fluor 488 ab150113, by Abcam (1 mentions)
Streptomycin, by Biowest (1 mentions)
N hexane, by Merck Group (1 mentions)
Diethyl phthalate, by Merck Group (1 mentions)
Dibutyl phthalate, by Merck Group (1 mentions)
Dimethyl phthalate, by Merck Group (1 mentions)
Diisobutyl phthalate, by Merck Group (1 mentions)
Bis 2 ethylhexyl phthalate, by Merck Group (1 mentions)
2 protocols using hplc grade petroleum ether
1
Melanogenic Pathway Regulation Analysis
2
Phthalate Esters Quantification Protocol
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We obtained dimethyl phthalate (DMP, ≥99%), dibutyl phthalate (DBP, 99%), di-isobutyl phthalate (DIBP, 99%), diethyl phthalate (DEP, 99.5%), and bis (2-ethylhexyl) phthalate (DEHP, ≥99%) from Sigma-Aldrich (Darmstadt, Germany). We purchased HPLC-grade petroleum ether and n-hexane from Merck Chemical Company (Darmstadt, Germany). We obtained analytical-grade H2SO4 (98.3%) from Shengqinghe Chemical Company (Nanjing, China). In addition, dealing with concentrated sulfuric acid and use special acid-resistant gloves and glasses
We used ultrapure water purified using a Millipore Purification System (Burlington, NJ, USA) in all experiments. To minimize the risk of contamination, we used glassware in all experiments, which we strictly washed and checked to ensure the lack of contamination with PAEs before use.
We prepared individual standard stock solutions of each phthalic acid ester (100 mmol L−1) and stored them at 4 °C. We prepared serial dilutions of individual standard stocks and multicomponent solutions containing equal concentrations (100 µmol L−1) of each compound on site by diluting the stock solution with methanol. We constructed calibration curves by plotting the means of triplicate measurements of peak highs against the concentrations of each compound.
We used ultrapure water purified using a Millipore Purification System (Burlington, NJ, USA) in all experiments. To minimize the risk of contamination, we used glassware in all experiments, which we strictly washed and checked to ensure the lack of contamination with PAEs before use.
We prepared individual standard stock solutions of each phthalic acid ester (100 mmol L−1) and stored them at 4 °C. We prepared serial dilutions of individual standard stocks and multicomponent solutions containing equal concentrations (100 µmol L−1) of each compound on site by diluting the stock solution with methanol. We constructed calibration curves by plotting the means of triplicate measurements of peak highs against the concentrations of each compound.
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