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2 protocols using atractylenolide 3

1

HPLC Analysis of Bioactive Compounds

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Methanol, acetonitrile, and chloroform (HPLC grade) were purchased from Fisher Chemical Co., Inc. (Geel, Belgium). Lobetyolin (reference standard, CAS No. 136085-37-5), syringin (CAS No. 118-34-3) and atractylenolide III (CAS No. 73030-7-14) was purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). All other chemicals and solvents were analytical grade and purchased from common sources.
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2

Quantification of Bioactive Compounds in LGZGD

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Liquiritin, isoLiquiritin, cinnamic acid, glycyrrhizic acid, coumarin, cinnamic alcohol, cinnamaldehyde, and atractylenolide III were obtained from Shanghai Yuanye Bio-Technology Company (Shanghai, China). The purity of all standard compounds has met the analytical requirements (>98%). The four herbs of LGZGD were purchased from Anhui Puren Chinese Medicine Co., Ltd. (Bozhou China). Acetonitrile and methanol were of chromatographic grades (Merck Company, Germany). Rat IL-1β ELISA kit was purchased from Meimian (Jiangsu, China). Antibodies (NLRP3, GSDMD, GAPDH) were purchased from Abcam (Cambridge, MA, USA). Caspase-1 p20 was purchased from Affinity Biosciences (Cincinnati, OH, USA).
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