U 87 mg atcc htb 14 homo sapiens brain

The human GBM cell lines U-138MG (U-138 MG ATCC® HTB-16™ Homo sapiens brain glioblastoma) and U-87 MG (U-87 MG ATCC^® HTB-14™ Homo sapiens brain Likely glioblastomas) were obtained from the ATCC (American Type Culture Collection, Rockville, MD, USA). Cell lines U-138 and U-87 were derived from human malignant gliomas and are commonly used as experimental models of glioblastoma13,14 (link). U-138 differs from U-87 in morphology, invasion, and proliferation rate13 (link). U-138 and U-87 exhibit different protein expression profiles, mainly characterized by a very low expression of glial fibrillary acidic protein (GFAP), generally expressed in glial cells and almost completely absent in U-87 cells15,16 (link). GBM cells lines were cultured in a 75-cm² flask with, respectively, ATCC-formulated Eagle’s Minimum Essential Medium (Catalog No. 30-2003; ATCC) for U-138 MG and Dulbecco’s modified Eagle’s medium (DMEM; Catalog No. D5030; Sigma-Aldrich, St. Louis, MO, USA) for U-87 MG, both supplemented with antibiotics (penicillin, 1000 U; streptomycin, 0.1 mg/L; Catalog No. P4333; Sigma-Aldrich), l-glutamine (GlutaMAX™, Catalog No. 35050061; ThermoFisher Scientific, Waltham, MA, USA), and 10% (v/v) fetal bovine serum (FBS; Catalog No. 12103C; Sigma-Aldrich) in a humidified atmosphere containing 5% CO₂ at 37° C.