Log In Sign up
The largest database of trusted experimental protocols

Incb024360

Manufactured by Selleck Chemicals
Visit Supplier

INCB024360 is a small molecule compound used in laboratory research. It functions as a selective inhibitor of the enzyme indoleamine 2,3-dioxygenase (IDO1). IDO1 is an enzyme involved in the metabolism of the amino acid tryptophan.

Automatically generated - may contain errors

Lab products found in correlation

Mccoy s 5a, by American Type Culture Collection (2 mentions) Recombinant human ifn γ, by Merck Group (2 mentions) Mccoy s 5a, by Thermo Fisher Scientific (2 mentions) Incb024360, by Merck Group (2 mentions) Epacadostat, by Selleck Chemicals (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Celltrace violet, by Thermo Fisher Scientific (1 mentions) Cd11c apc, by Miltenyi Biotec (1 mentions) Interleukin 3 (il 3), by Thermo Fisher Scientific (1 mentions) Wst 1 cell proliferation reagent, by Takara Bio (1 mentions) Dcp mm medium, by MatTek (1 mentions) Cd3 pe fitc apc, by BD (1 mentions) Cd8 apc fitc, by BD (1 mentions) Cd123 pe pe cy5 fitc, by BD (1 mentions) Cd138 fitc pe apc, by BD (1 mentions) Celltracker green flow assay kits, by Thermo Fisher Scientific (1 mentions) Ro 61 8048, by Selleck Chemicals (1 mentions) Pd l1 bv421, by BioLegend (1 mentions) Bdca 2 fitc, by Miltenyi Biotec (1 mentions)

4 protocols using incb024360

1

Inhibitors Modulate Kynurenine in Tumor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
SKOV3 human ovary adenocarcinoma (IDO1+) and A172 human glioblastoma (TDO+) cell lines were obtained from ATCC and cultured in McCoy’s 5A (10% FBS) and Dulbecco’s Modified Eagle’s Medium (DMEM, 10% FBS) culture media, respectively. Cell lines were incubated at 37°C in 96 well plates with either the IDO1 inhibitor INCB024360 (Epacadostat, Selleck) at 2 μM for SKOV3 cells and 20 μM for A172 cells; the TDO inhibitor 680C91 (WuXi Apptec) at 20 μM for all cells; or Kynureninase (Mp-KYNase) at 0.36 mg/ml for all cells. In addition, A172 cells were stimulated with recombinant human IFNγ (100 ng/mL, Sigma) to induce IDO1 expression to determine the effects on Kynureninase, INCB024360 and 680C91 on Kyn levels made by IDO1/TDO dual expressing cells. After 48 h, cell supernatants were collected, snap frozen in liquid nitrogen and stored at −80°C. Kynurenine concentrations of the supernatant were then determined by LC/MS. McCoy’s 5A, DMEM and FBS were all purchased from GIBCO®.
Triplett T.A., Garrison K.C., Marshall N., Donkor M., Blazeck J., Lamb C., Qerqez A., Dekker J.D., Tano Y., W.e.i.-.C.h.e.n.g.-.L.u., Karamitros C.S., Ford K., Tan B., Zhang M., McGovern K., Coma S., Kumada Y., Yamany M.S., Sentandreu E., Fromm G., Tiziani S., Schreiber T.H., Manfredi M., Ehrlich L.I., Stone E, & Georgiou G. (2018). Reversal of IDO-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nature biotechnology, 36(8), 758-764.
+ Open protocol
+ Expand
2

Investigating MM-pDC Interactions and Immunotherapies

Check if the same lab product or an alternative is used in the 5 most similar protocols
MM cells were cultured in 10% FBS plus RPMI-1640 medium supplemented with antibiotics. MM–pDCs were cocultured either in DCP-MM medium (Mattek Corp., Ashland, MA) or complete RPMI-1640 medium supplemented with IL-3 (Peprotech Inc., Rocky Hill, NJ, USA). CD3-PE/FITC/APC; CD4-FITC/PE or APC-Cy7; CD8-APC/FITC, CD56-PE; CD123-PE/PE-Cy5/FITC; and CD138-FITC/PE/APC were obtained from BD Biosciences (San Jose, CA). BDCA-2-FITC and CD11c-APC were obtained from Miltenyi Biotec (Auburn, CA); CD303-; CD304-; CD107a; and PD-L1-BV421 were purchased from Biolegend. All immunomagnetic separation kits were purchased from Miltenyi Biotec. The CellTrace Violet and CellTracker Green flow assay kits were obtained from Life Technologies (USA). Functional-grade PD-L1 blocking antibody (antihuman PD-L1, clone MIH1) was obtained from eBiosciences [4 (link)]. Ro 61–8048 [13 (link)] and INCB 024360 were purchased from Selleck Chemicals. WST-1 Cell Proliferation Reagent was purchased from Clontech Laboratories, Inc. (USA).
Ray A., Song Y., Du T., Tai Y.T., Chauhan D, & Anderson K.C. (2019). Targeting tryptophan catabolic kynurenine pathway enhances antitumor immunity and cytotoxicity in multiple myeloma. Leukemia, 34(2), 567-577.
+ Open protocol
+ Expand
3

Inhibitors Modulate Kynurenine in Tumor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
SKOV3 human ovary adenocarcinoma (IDO1+) and A172 human glioblastoma (TDO+) cell lines were obtained from ATCC and cultured in McCoy’s 5A (10% FBS) and Dulbecco’s Modified Eagle’s Medium (DMEM, 10% FBS) culture media, respectively. Cell lines were incubated at 37°C in 96 well plates with either the IDO1 inhibitor INCB024360 (Epacadostat, Selleck) at 2 μM for SKOV3 cells and 20 μM for A172 cells; the TDO inhibitor 680C91 (WuXi Apptec) at 20 μM for all cells; or Kynureninase (Mp-KYNase) at 0.36 mg/ml for all cells. In addition, A172 cells were stimulated with recombinant human IFNγ (100 ng/mL, Sigma) to induce IDO1 expression to determine the effects on Kynureninase, INCB024360 and 680C91 on Kyn levels made by IDO1/TDO dual expressing cells. After 48 h, cell supernatants were collected, snap frozen in liquid nitrogen and stored at −80°C. Kynurenine concentrations of the supernatant were then determined by LC/MS. McCoy’s 5A, DMEM and FBS were all purchased from GIBCO®.
Triplett T.A., Garrison K.C., Marshall N., Donkor M., Blazeck J., Lamb C., Qerqez A., Dekker J.D., Tano Y., W.e.i.-.C.h.e.n.g.-.L.u., Karamitros C.S., Ford K., Tan B., Zhang M., McGovern K., Coma S., Kumada Y., Yamany M.S., Sentandreu E., Fromm G., Tiziani S., Schreiber T.H., Manfredi M., Ehrlich L.I., Stone E, & Georgiou G. (2018). Reversal of IDO-mediated cancer immune suppression by systemic kynurenine depletion with a therapeutic enzyme. Nature biotechnology, 36(8), 758-764.
+ Open protocol
+ Expand
4

Modulation of T Cell Proliferation by CD14-CD15+ Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD14−CD15+ cells were purified from PBMCs or TNF-α-treated PBMCs with CD14-negative and CD15-positive selection kits (Miltenyi Biotec). Additionally, CFSE-labeled (Invitrogen) Pan T cells were purified from HC using magnetic beads (Miltenyi Biotec). The two sets of purified cells were co-cultured in various ratios and stimulated with human T-activator CD3/CD28 (eBscience) and/or IDO inhibitor (INCB024360, Selleck) for 3 days. Cells were stained with PE-Cy7 anti-human CD8 (eBscience), PE anti-human CD4 (eBscience), and FITC anti-human CD3 (eBscience). T cell proliferation was analyzed using flow cytometry.
For intracellular cytokine detection, co-cultured cells were stimulated with 50 ng/mL PMA (Sigma-Aldrich), 1 μg/mL ionomycin (Sigma-Aldrich), and 5 μg/mL BFA (Sigma-Aldrich) for 5 h. T cells were permeabilized and stained with APC anti-human IFN-γ (eBscience), PE anti-human IL-2 (eBscience), and PE-Cy7 anti-human TNF-α (eBscience).
Yu X., Sun J., Yang F., Mao R., Shen Z., Ren L., Yuan S., He Q., Zhang L., Yang Y., Ding X., He Y., Zhu H., Shen Z., Zhu M., Qiu C., Su Z, & Zhang J. (2023). Granulocytic myeloid-derived suppressor cells increase infection risk via the IDO/IL-10 pathway in patients with hepatitis B virus-related liver failure. Frontiers in Immunology, 13, 966514.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!