Pectin

Phanta max super-fidelity DNA polymerase (P505-d1) was purchased from Vazyme (Nanjing, China). Takara quick-cut enzyme and T4 DNA ligase kit were purchased from Biomedical Technology Co., Ltd. (Beijing, China). Chondroitin sulfate A (CS-A), CS-B, and CS-C were purchased from Hefei Bomei Biotechnology Co., Ltd. (Hefei, China). HA, alginate, pectin, xanthan, and heparin were purchased from Solarbio (Beijing, China). TIANamp bacteria DNA kit (Tiangen, China) was used to extract the bacterial genome. HisTrap HP column and Superdex Peptide 10/300 GL was purchased from GE Healthcare (Pittsburgh, PA, USA).

Escherichia coli DH5α, P. pastoris X-33 were used as host for plasmid propagation and heterologous gene expression. Wheat arabinoxylan was purchased from Megazyme International (Irish, Ireland). Beechwood xylan was purchased from Sigma-Aldrich (St. Louis, MO, United States). Oat pelt xylan, pectin, and sodium carboxymethyl cellulose were purchased from Solarbio (Bejing, China). The restriction endonucleases were purchased from Thermo Fisher Scientific (Shanghai, China). Wholewheat flour with 57% water absorption, 11.00% protein, and 12.10% moisture was purchased from Saixin Flour Industry Co. Ltd. (Bayannur, China). The buffered methanol-complex and glycerol-complex medium (BMMY/BMGY), yeast extract peptone dextrose agar medium (YPD), and low salt Luria Bertani media (LBL) were prepared according to the yeast fermentation processing guidelines (Invitrogen). Unless otherwise stated, other reagents were analytical pure reagents, purchased from China.