Human haptoglobin

Human haptoglobin (from pooled human plasma, a mixture of Hp1-1, 2-1, 2-2, Cat #3536), triton X-114, imidazole, human macrophage-colony stimulating factor (M-CSF), acetaminophen and dexamethasone (Cat # D2915) were purchased from Sigma (St. Louis, MO). NHS-activated sepharose 4 fast flow beads were obtained from GE Healthcare (Cat #17-0906-01, Uppsala, Sweden). Trizol reagent was from Life Technologies (Carlsbad, CA). The RevertAid™ First Strand cDNA Synthesis Kit was from Fermentas (Vilnius, Lithuania). SYBR Premix Ex Taq™ II was from TaKaRa Bio Inco (Otsu, Japan). Thioglycollate medium was purchased from Becton Dickinson Co., (Sparks, MD). Primers for QPCR, trypsin-EDTA and carbenicillin were from Invitrogen Inc., (Carlsbad, CA). Isopropyl-D-thiogalactopyranoside (IPTG) was purchased from Pierce (Rockford, IL). Recombinant human CD163 protein, CD163 expression plasmid (SC117495, NM_004244.3) and MegaTran 1.0 transfection reagent were purchased from Origene (Rockville, MD). Antibodies for human heme oxigenase-1 (HO-1, Cat # ab52946) and CD163 (Cat # MCA1853) were from ABcam (Cambridge, MA) and Serotec (Raleigh, NC) respectively. Anti-human CD163-PE and anti-human CD80-PE antibodies were from BioLegend (San Diego, CA). FITC antibody labeling kit is from Thermo Scientific (Rockford, IL). Alanine transaminase (ALT) assay kits were from BIOO Scientific Corp., (Austin, TX).